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Application of antimicrobials for the elimination of Escherichia coli and Listeria monocytogenes in brine injected beef

  • Author / Creator
    Ward, Patrick J
  • The application of antimicrobials to a brine-injected raw beef roast for the elimination/inhibition of heat resistant Escherichia coli and Listeria monocytogenes was investigated. The choice of antimicrobials for use in brine injected beef was based on minimum bactericidal concentration in brine solutions. Charsol® and MicocinX™, were added to the brine solution individually and in combination to evaluate possible synergy between the antimicrobials. In the brine solution, numbers of E. coli were not reduced by either antimicrobial; however, L. monocytogenes was reduced by more than 2 log CFU/mL in the presence of either antimicrobial, and no synergistic effect was detected. For the brine-injected beef, neither antimicrobial had any effect on numbers of E. coli, and the counts remained the same during 7 days of storage at 7°C. L. monocytogenes was unaffected by the Charsol®; however, counts were reduced when MicocinX™ was present and growth was inhibited during 4 days of storage.

  • Subjects / Keywords
  • Graduation date
    2013-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3M61BX42
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Agricultural, Food, and Nutritional Science
  • Specialization
    • Food Science and Technology
  • Supervisor / co-supervisor and their department(s)
    • McMullen, Lynn (Agricultural, Food and Nutritional Science)
    • Nattress, Frances (Agricultural, Food and Nutritional Science)
    • Gaenzle, Michael (Agricultural, Food and Nutritional Science)
  • Examining committee members and their departments
    • Gaenzle, Michael (Agricultural, Food and Nutritional Science)
    • Betti, Mirko (Agricultural, Food and Nutritional Science)
    • Nattress, Frances (Agricultural, Food and Nutritional Science)
    • McMullen, Lynn (Agricultural, Food and Nutritional Science)