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Regulation of vertebrate ladybird genes

  • Author / Creator
    Lukowski, Chris
  • Development of the vertebrate central nervous system is a complex process that relies on the accurate spatiotemporal distribution of signaling centers during embryogenesis. These signals provide cells with positional information, which is integrated via transcription factors and gene regulatory elements to generate a specific downstream gene expression profile that confers specific cellular functions. It is of interest to determine how cells acquire their unique spatiotemporal gene expression patterns. The wide variety of expression profiles established along the dorsoventral axis of the neural tube provides a great system to address this question. Recent advances in zebrafish transgenic technology, along with the phenomenon of a fish-specific genome duplication event, have been exploited here to provide an efficient way of identifying and characterizing gene regulatory elements. An identified neuronal-specific enhancer near the ladybird locus has been incorporated into a transgenic zebrafish strain driving fluorescent reporter protein expression in a subset of dorsal interneurons

  • Subjects / Keywords
  • Graduation date
    2010-06
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3S32S
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Biological Sciences
  • Supervisor / co-supervisor and their department(s)
    • Waskiewicz, Andrew (Biological Sciences)
  • Examining committee members and their departments
    • Allison, William (Biological Sciences)
    • Underhill, Alan (Medical Genetics)
    • Pilgrim, David (Biological Sciences)