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Characterization of Human Pancreatic Beta-cell Progenitors as a Means to Alleviate the Shortage of Donor Tissue for Islet Transplantation

  • Author / Creator
    Anderson, Sarah J
  • The current shortage of islet tissue from cadaveric human donors is not sustainable and will preclude islet transplantation from becoming a widespread therapeutic treatment for those with type 1 diabetes. A potential alternative source of tissue may be through isolating, expanding, and differentiating beta-cell progenitors within the adult human pancreas. We sought to characterize the population of origin of beta-cell progenitors. Our human studies use pancreatic beta-cell progenitor marker, Pdx-1 to study the development and origin of beta-cells. In vivo immunohistochemical study of fetal development demonstrates morphologic evolution of islets throughout maturation and expression and interaction of several factors important to beta-cell development. In vitro study utilizes a novel cell culture media permitting long-term study of the epithelial population within the adult pancreas as well as lentiviral reporters and immunostaining to observe the differentiation of potential progenitor cells. Together our data suggests that Pdx-1 expressing progenitor cells are epithelial cells.

  • Subjects / Keywords
  • Graduation date
    2012-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3D077
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Surgery
  • Specialization
    • Experimental Surgery
  • Supervisor / co-supervisor and their department(s)
    • Korbutt, Gregory (Surgery)
  • Examining committee members and their departments
    • Churchill, Thomas (Surgery) (Chair)
    • Bell, Rhonda (Agricultural, Food and Nutritional Science)
    • MacDonald, Patrick (Pharmacology)