The Na+/H+ exchanger potentiates growth and retinoic acid induced differentiation of embryonal carcinoma cells

• Author(s) / Creator(s)

  • Wang, Huayan
  • Singh, Dyal
  • Fliegel, Larry

• The Na+/H+exchanger is a ubiquitous plasma membrane protein that is responsible for pH regulation and is activated by growth factors. We examined the role of the Na+/H+ exchanger in cell growth and differentiation. Treatment of P19 cells with the Na+/H+ exchanger inhibitor Hoe 694 eliminated retinoic acid-induced differentiation in this cell line. We developed a P19 embryonal carcinoma cell line that was deficient in the Na+/H+ antiporter. Na+/H+ exchanger-deficient cells were reduced in the rate of cell growth and this effect was enhanced by the removal of added HCO3 − and by reducing extracellular pH. The antiporter-deficient cells were also markedly deficient in their ability to differentiate to neuronal-like cells and recovered this ability when the Na+/H+antiporter was reintroduced. The results show that the absence of Na+/H+ antiport as a pH regulatory mechanism can result in deficiencies in both cell growth and differentiation in embryonal carcinoma cells.

• Date created

  1997-01-01

• Subjects / Keywords

  • Intracellular Ph Regulation
  • Hl-60 Cells
  • Leukemic-Cells
  • Activation
  • Proximal Tubules
  • Proliferation
  • Exchanger Gene
  • Gene-Expression
  • Monocytic Differentiation
  • Smooth-Muscle

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/r3-17dr-yf75

• License

  Attribution 4.0 International