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Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish

  • Author / Creator
    Brewster, Daniel L
  • The escape response in zebrafish is mediated in part by the Mauthner cell and its two homologues, MiD2cm and MiD3cm. In adult fish, the Mauthner cell fires a single action potential when activated while the homologues fire multiple action potentials. Their distinct firing properties are partially attributed to the differential expression of voltage gated potassium (Kv) channels. I am interested in determining the Kv channels associated with the Mauthner and MiD2cm cells, and if they contain Kv1.1. Immunohistochemistry and in situ hybridization confirmed the expression of Kv1.1 in the Mauthner and MiD2cm cells at 48 hours post fertilization. Electrophysiology showed these cells to contain A-type and delayed rectifier currents but with different current densities. Preliminary data has shown these cells have the same firing behavior as in adults but with distinct action potential waveforms. The presence of Kv1.1 and the distinct current properties are suspected of regulating the neuronal excitability.

  • Subjects / Keywords
  • Graduation date
    2012-06
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R31C1TQ2N
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Biological Sciences
  • Specialization
    • Physiology, Cell and Developmental Biology
  • Supervisor / co-supervisor and their department(s)
    • Ali, Declan (Biological Sciences)
  • Examining committee members and their departments
    • Dickson, Clayton (Psychology)
    • Goss, Greg (Biological Sciences)
    • Gallin, Warren (Biological Sciences)