Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish

  • Author / Creator
    Brewster, Daniel L
  • The escape response in zebrafish is mediated in part by the Mauthner cell
    and its two homologues, MiD2cm and MiD3cm. In adult fish, the Mauthner cell
    fires a single action potential when activated while the homologues fire multiple action potentials. Their distinct firing properties are partially attributed to the differential expression of voltage gated potassium (Kv) channels. I am interested in determining the Kv channels associated with the Mauthner and MiD2cm cells, and if they contain Kv1.1. Immunohistochemistry and in situ hybridization confirmed the expression of Kv1.1 in the Mauthner and MiD2cm cells at 48 hours post fertilization. Electrophysiology showed these cells to contain A-type and
    delayed rectifier currents but with different current densities. Preliminary data has
    shown these cells have the same firing behavior as in adults but with distinct
    action potential waveforms. The presence of Kv1.1 and the distinct current properties are suspected of regulating the neuronal excitability.

  • Subjects / Keywords
  • Graduation date
    Spring 2012
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
  • Specialization
    • Physiology, Cell and Developmental Biology
  • Supervisor / co-supervisor and their department(s)
  • Examining committee members and their departments
    • Gallin, Warren (Biological Sciences)
    • Dickson, Clayton (Psychology)
    • Goss, Greg (Biological Sciences)