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Structural and functional studies of the core splicing factor Prp8 Open Access


Other title
Type of item
Degree grantor
University of Alberta
Author or creator
Wu, Tao
Supervisor and department
MacMillan, Andrew (Biochemistry)
Examining committee member and department
Owttrim, George (Biological Sciences)
MacMillan, Andrew (Biochemistry)
Glover, Mark J.N. (Biochemistry)
Kothe, Ute (Chemistry and Biochemistry, University of Lethbridge)
Schultz, Michael C. (Biochemistry)
Department of Biochemistry

Date accepted
Graduation date
Doctor of Philosophy
Degree level
More than 90% of human genes undergo a processing step called splicing, whereby noncoding introns are removed from initial transcripts and coding exons are ligated together to yield mature messenger RNA. Splicing involves two sequential transesterification reactions catalyzed by a large RNA/protein complex called the spliceosome. RNA components of the spliceosome are at least partly responsible for splicing catalysis. However, the precise architecture of the spliceosome active site and whether it includes protein components remain unknown. Numerous genetic and biochemical analyses have placed one of the largest and most highly conserved of nuclear proteins, Prp8, at the heart of the catalytic core of the splicing machinery during spliceosome assembly and through catalysis. Here we provide structural and functional evidence that the RNase H domain of Prp8 undergoes a conformational change during splicing which unmasks a metal-binding site required for the second step of splicing. We are able to demonstrate that a magnesium ion essential for the catalysis binds to the RNase H domain of Prp8 and a metal specificity switch which abrogates metal binding severely inhibits the second step of splicing. We also show that yeast prp8 first- and second-step alleles correspond to Prp8 mutants that favour one of the two distinct Prp8 conformations observed in the crystal structure of Prp8 RNase H domain. Together these data support the model of rearrangements within the spliceosome at the time of transition between the first and second step of splicing. Our findings also establish that Prp8 is a metalloprotein which promotes exon ligation and are consistent with the designation of the spliceosome as a ribonucleoprotein enzyme.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Chilibeck et al., (2006) J. Biol. Chem.

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