Dietary Gangliosides Modify Helicobacter pylori Adhesion to Human Gastric Epithelial Cells Open Access
- Other title
Gangliosides alter apical gastric membranes
Gangliosides decrease H pylori adhesion
- Type of item
- Degree grantor
University of Alberta
- Author or creator
Rivas Serna, Irma M
- Supervisor and department
Clandinin, Tom (AFNS)
- Examining committee member and department
Keelan, Monika (Laboratory of Medicine and Pathology)
Schnabl, Kareena (U of A Hospital)
Lynn McMullen (AFNS)
Jones, Peter (Food Science U of M)
Mazurak, Vera (AFNS)
Clandinin, Tom (AFNS)
Department of Agricultural, Food, and Nutritional Science
Nutrition and Metabolism
- Date accepted
- Graduation date
Doctor of Philosophy
- Degree level
This research determined if alteration of plasma membrane by exogenous gangliosides inhibits Helicobacter pylori adhesion to human gastric epithelial cells. The objective was to evaluate the composition of gangliosides from bovine whole raw milk, bovine colostrum and powder buttermilk using Liquid Chromatography/Mass Spectrometry (LC/MS). This research determined if alteration of the GM3 and GD3 content of the apical and basolateral membrane of a human gastric cell line was modified by exogenous gangliosides. The effect of ganglioside treatment on the composition and content of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) and on H. pylori adhesion was assessed.
A rapid and sensitive LC/MS method was established and used to determine content and composition of gangliosides. Ganglioside composition was analyzed in three different milk products (raw milk, colostrum, buttermilk). GD3 was found to be the most abundant ganglioside species. Human gastric cells fed with exogenous GM3 and GD3 modified ganglioside content, but most abundant ganglioside was GM3. GM3 was found in the apical side and GD3 in the basolateral side of the cell. After ganglioside treatments, composition of ganglioside content of membrane domains was modified. Composition and content of phospholipids was not altered after ganglioside treatment. Gastric cells treated with gangliosides decreased H. pylori adhesion, but concentration of these gangliosides was not crucial for reduction. GM3 and GD3 decrease H. pylori adhesion. The combination of GM3:GD3 also decreased H. pylori adhesion suggesting that human and bovine milk might decrease H. pylori adhesion.
This thesis demonstrates that 1) gangliosides are taken up in gastric cells, 2) gangliosides fed from the apical side also affect content of gangliosides in the basolateral side, and 3) GD3 and GM3 reduce H. pylori adhesion to human gastric cells. This research suggests that gangliosides may be an anti-adhesion strategy to decrease or prevent H. pylori infection.
- Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
- Citation for previous publication
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 3073530
Last modified: 2015:10:22 06:05:35-06:00
Original checksum: 6f6b71f7c999745b4b8e1f826eb8a0cf
Activity of users you follow