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Co-transplantation of neonatal porcine islets with Sertoli cells combined with short-term monoclonal antibody therapy in preventing neonatal porcine islet xenograft rejection Open Access


Other title
Anti-CD154 monoclonal antibody
Sertoli cells
Neonatal porcine islets
Anti-LFA-1 monoclonal antibody
Islet xenotransplantation
Anti-CD45RB monoclonal antibody
Type of item
Degree grantor
University of Alberta
Author or creator
Ramji, Qahir A.
Supervisor and department
Rayat, Gina (Surgery)
Examining committee member and department
Rajotte, Ray (Surgery)
Field, Catherine (Agricultural, Food and Nutritional Science)
Agrawal, Babita (Surgery)
Department of Surgery

Date accepted
Graduation date
Master of Science
Degree level
The need for an unlimited source of islets and a safer method of immunosuppression has limited the widespread application of islet transplantation. To remedy the shortage of donor tissue, xenotransplantation of neonatal porcine islets (NPI) has been proposed. In this study we sought to determine if combining co-transplantation of NPI with Sertoli cells (SC) with a short-term monoclonal antibody (mAb) therapy would prevent NPI xenograft rejection. We hypothesize that this combination of treatments will lead to long-term NPI xenograft survival. Our result show a significant increase in the proportion of mice achieving long-term graft survival compared to untreated mice transplanted with NPI alone, as 7/7 mice treated with anti-LFA-1 mAb (p=0.001), 7/8 mice treated with anti-CD154 mAb (p=0.003), and 4/9 mice treated with anti-CD45RB mAb (p=0.020) achieved and maintained normoglycemia long-term. Therefore, we conclude that the combination of mAb therapy with SC is highly efficacious in preventing NPI xenograft rejection.
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