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The Role of Rho GTPases, Rac1 and Rac2, in Mast Cell Exocytosis Open Access


Other title
Rac1 and Rac2 GTPases
mast cell
Type of item
Degree grantor
University of Alberta
Author or creator
Baier, Alicia
Supervisor and department
Eitzen, Gary (Cell Biology)
Examining committee member and department
LaPointe, Paul (Cell Biology)
Lacy, Paige (Medicine)
Vliagoftis, Harissios (Medicine)
Department of Cell Biology

Date accepted
Graduation date
Master of Science
Degree level
Mast cells are tissue-resident immune cells that undergo exocytosis upon activation, releasing potent immunoregulatory molecules that initiate inflammatory responses. Here, I investigated the role of the hematopoietic specific Rho GTPase, Rac2, and the ubiquitous, Rac1, on mast cell exocytosis. Our hypothesis is that Fc(epsilon)RI-mediated mast cell exocytosis is differentially regulated by Rac1 and Rac2 GTPases. Three experimental systems were used to investigate their roles in mast cell function; Rac2 knockout mice, Rac1 and Rac2 siRNA knockdown, and treatment with the Rac inhibitor, EHT-1864. My results suggest that Rac1 and Rac2 regulate multiple stages of Fc(epsilon)RI-mediated response in mast cells. I found that calcium flux and exocytosis were Rac1 and Rac2-dependent processes. My data suggests that Rac1 and Rac2 regulate exocytosis through a convergent pathway but regulate calcium flux through alternate pathways. Moreover, I found that Rac2 does not regulate actin remodelling, suggesting this may be a Rac1-mediated process.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: Alicia Baier M. Sc. THESIS prefatory pages for FGSR, 2012.pdf
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