Journal Articles (Chemistry)

2010

El-Hawiet, Amr, Liu, Lan, Klassen, John S., Kitova, Elena N.

A new electrospray ionization mass spectrometry (ES-MS) approach for quantifying protein—ligand complexes that are prone to in-source (gas-phase) dissociation is described. The method, referred to here as the reference ligand ES-MS method, is based on the direct ES-MS assay and competitive ligand...

2015

N. Kitova, Elena N., Shams-Ud-Doha, Km, Yao, Yuyu, Klassen, John S., Daneshfar, Rambod

The application of liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) for quantifying protein–carbohydrate interactions in vitro is described. Association constants for the interactions between lysozyme and β-D-GlcNAc-(1 → 4)-β-D-GlcNAc-(1 → 4)-D-GlcNAc and...

2015

Klassen, John S., N. Kitova, Elena N., Yao, Yuyu, Shams-Ud-Doha, Km, Daneshfar, Rambod

The application of liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) for quantifying protein–carbohydrate interactions in vitro is described. Association constants for the interactions between lysozyme and β-D-GlcNAc-(1 → 4)-β-D-GlcNAc-(1 → 4)-D-GlcNAc and...

2011

Liu, Lan, Kitova, Elena N., Klassen, John S.

The application of the direct electrospray ionization mass spectrometry (ESI-MS) assay to quantify interactions between bovine β-lactoglobulin (Lg) and a series of fatty acids (FA), CH3(CH2)xCOOH, where x = 6 (caprylic acid, CpA), 8 (capric acid, CA), 10 (lauric acid, LA), 12 (myristic acid, MA),...

2011

Klassen, John S., Kitova, Elena N., Liu, Lan

The application of the direct electrospray ionization mass spectrometry (ESI-MS) assay to quantify interactions between bovine β-lactoglobulin (Lg) and a series of fatty acids (FA), CH3(CH2)xCOOH, where x = 6 (caprylic acid, CpA), 8 (capric acid, CA), 10 (lauric acid, LA), 12 (myristic acid, MA),...

2014-01-01

Heshmatollah Ebrahimi-Najafabadi,Teague M. McGinitie, James J. Harynuk

Thermodynamic parameters of ∆H(T0), ∆S(T0), and ∆Cp for 156 compounds comprising alkanes, alkyl halides and alcohols were determined for a 5% phenyl 95% methyl stationary phase. The determination of thermodynamic parameters relies on a Nelder-Mead simplex optimization to rapidly obtain the...

2013-01-01

Teague M. McGinitie, Heshmatollah Ebrahimi-Najafabadi, and James J. Harynuk

A new method for estimating the thermodynamic parameters of ΔH(T0), ΔS(T0), and ΔCP for use in thermodynamic modeling of GC×GC separations has been developed. The method is an alternative to the traditional isothermal separations required to fit a three-parameter thermodynamic model to retention...

2015

El-Hawiet, Amr, N. Kitova, Elena N., Klassen, John S.

The affinities of the most abundant oligosaccharides found in human milk for four bacterial exotoxins (from Vibrio cholerae and pathogenic Escherichia coli) were quantified for the first time. Association constants (Ka) for a library of 20 human milk oligosaccharides (HMOs) binding to Shiga toxin...

2015

El-Hawiet, Amr, Klassen, John S., N. Kitova, Elena N.

The affinities of the most abundant oligosaccharides found in human milk for four bacterial exotoxins (from Vibrio cholerae and pathogenic Escherichia coli) were quantified for the first time. Association constants (Ka) for a library of 20 human milk oligosaccharides (HMOs) binding to Shiga toxin...

2013-01-01

Parasuraman, Deepika, Sarker, Avijeet K., Serpe, Michael J.

Poly (N-isopropylacrylamide)-co-acrylic acid (pNIPAm-co-AAc) microgels and their aggregates have been shown to effectively remove organic dye molecules from aqueous solutions. Here, we investigate the reusability of these microgel-based systems by exposing them to the organic azo dye molecule,...