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Journal Articles (Chemistry)
Items in this Collection
- 20Electrospray ionization mass spectrometry
- 12Affinity
- 10Carbohydrates
- 9Complexes
- 6Binding
- 6Mass-spectrometry
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Screening oligosaccharide libraries against lectins using the proxy protein electrospray ionization mass spectrometry assay
Download2016
Kitova, Elena N., Klassen, John S., Fan, Xuxin, Privé, Gilbert G., Cairo, Christopher W., Xiong, Zi Jian, Ng, Kenneth K. S., Zou, Chunxia, Eugenio, Luiz, Li, Jun
An electrospray ionization mass spectrometry (ESI-MS) assay for screening carbohydrate libraries against lectins is described. The assay is based on the proxy protein ESI-MS method, which combines direct ESI-MS protein–ligand binding measurements and competitive protein binding, to simultaneously...
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Structural basis for antibody recognition in the receptor-binding domains of toxins A and B from Clostridium difficile
Download2014
Ng, Kenneth K. S., Tanha, Jamshid, Kitova, Elena N., Eugenio, Luiz, Murase, Tomohiko, Klassen, John S., Hussack, Greg, Schorr, Melissa
Clostridium difficile infection is a serious and highly prevalent nosocomial disease in which the two large, Rho-glucosylating toxins TcdA and TcdB are the main virulence factors. We report for the first time crystal structures revealing how neutralizing and non-neutralizing single-domain...
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Structure and stability of carbohydrate-lipid interactions: Methylmannose polysaccharide-fatty acid complexes
Download2016
Mayer, Paul M., Richards, Michele R., Lowary, Todd L., Renaud, Justin, Klassen, John S., Tieleman, D. Peter, Kitova, Elena N., Liu, Lan, Siuda, Iwona
We report a detailed study of the structure and stability of carbohydrate–lipid interactions. Complexes of a methylmannose polysaccharide (MMP) derivative and fatty acids (FAs) served as model systems. The dependence of solution affinities and gas-phase dissociation activation energies (Ea) on FA...
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Structure and stability of carbohydrate-lipid interactions: Methylmannose polysaccharide-fatty acid complexes
Download2016
Klassen, John S., Siuda, Iwona, Renaud, Justin, Tieleman, D. Peter, Kitova, Elena N., Liu, Lan, Mayer, Paul M., Richards, Michele R., Lowary, Todd L.
We report a detailed study of the structure and stability of carbohydrate–lipid interactions. Complexes of a methylmannose polysaccharide (MMP) derivative and fatty acids (FAs) served as model systems. The dependence of solution affinities and gas-phase dissociation activation energies (Ea) on FA...
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Thermal dissociation of streptavidin homotetramer in the gas phase: Subunit loss versus backbone fragmentation
Download2013
Deng, Lu, Klassen, John S., Kitova, Elena N., Sinelnikov, Igor
The results of time-resolved blackbody infrared radiative dissociation experiments performed on gaseous protonated ions, at charge states +15, +16 and +17, of the homotetramer streptavidin (S4) are reported. Evidence is found for three dissociation pathways involving (i) the loss of a single...
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Thermal dissociation of streptavidin homotetramer in the gas phase: Subunit loss versus backbone fragmentation
Download2013
Klassen, John S., Sinelnikov, Igor, Kitova, Elena N., Deng, Lu
The results of time-resolved blackbody infrared radiative dissociation experiments performed on gaseous protonated ions, at charge states +15, +16 and +17, of the homotetramer streptavidin (S4) are reported. Evidence is found for three dissociation pathways involving (i) the loss of a single...
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2015
Zhong, Weiming, Quigley, Christina, Klassen, John S., Fang, Hao, Jiang, Xi, Wei, Chao, Tan, Ming, Xia, Ming, Huang, Pengwei, Fan, Qiang, Zhang, Xufu
The recent discovery that human noroviruses (huNoVs) recognize sialic acids (SAs) in addition to histo-blood group antigens (HBGAs) pointed to a new direction in studying virus-host interactions during calicivirus infection. HuNoVs remain difficult to study due to the lack of an effective cell...