Tulane virus recognizes sialic acids as cellular receptors

  • Author(s) / Creator(s)
  • The recent discovery that human noroviruses (huNoVs) recognize sialic acids (SAs) in addition to histo-blood group antigens (HBGAs) pointed to a new direction in studying virus-host interactions during calicivirus infection. HuNoVs remain difficult to study due to the lack of an effective cell culture model. In this study, we demonstrated that Tulane virus (TV), a cultivable primate calicivirus, also recognizes SAs in addition to the previously known TV-HBGA interactions. Evidence supporting this discovery includes that TV virions bound synthetic sialoglycoconjugates (SGCs) and that treatment of TV permissive LLC-MK2 cells with either neuraminidases or SA-binding lectins inhibited TV infectivity. In addition, we found that Maackia amurensis leukoagglutinin (MAL), a lectin that recognizes the α-2,3 linked SAs, bound LLC-MK2 cells, as well as TV, by which MAL promoted TV infectivity in cell culture. Our findings further highlight TV as a valuable surrogate for huNoVs, particularly in studying virus-host interactions that may involve two host carbohydrate receptors or co-receptors for infection.

  • Date created
  • Subjects / Keywords
  • Type of Item
    Article (Published)
  • DOI
  • License
    Attribution 4.0 International
  • Language
  • Citation for previous publication
    • Tan, M., Xia, M., Wei, C., Fang, H., Quigley, C., Huang, P., Zhong, W., Zhang, X., Fan, Q., Klassen, J., and Jiang, X. (2015). Tulane virus recognizes sialic acids as cellular receptors. Scientific Reports, 5(July), 11784 [14 pages].
  • Link to related item