Tulane virus recognizes sialic acids as cellular receptors

• Author(s) / Creator(s)

  • Zhong, Weiming
  • Quigley, Christina
  • Klassen, John S.
  • Fang, Hao
  • Jiang, Xi
  • Wei, Chao
  • Tan, Ming
  • Xia, Ming
  • Huang, Pengwei
  • Fan, Qiang
  • Zhang, Xufu

• The recent discovery that human noroviruses (huNoVs) recognize sialic acids (SAs) in addition to histo-blood group antigens (HBGAs) pointed to a new direction in studying virus-host interactions during calicivirus infection. HuNoVs remain difficult to study due to the lack of an effective cell culture model. In this study, we demonstrated that Tulane virus (TV), a cultivable primate calicivirus, also recognizes SAs in addition to the previously known TV-HBGA interactions. Evidence supporting this discovery includes that TV virions bound synthetic sialoglycoconjugates (SGCs) and that treatment of TV permissive LLC-MK2 cells with either neuraminidases or SA-binding lectins inhibited TV infectivity. In addition, we found that Maackia amurensis leukoagglutinin (MAL), a lectin that recognizes the α-2,3 linked SAs, bound LLC-MK2 cells, as well as TV, by which MAL promoted TV infectivity in cell culture. Our findings further highlight TV as a valuable surrogate for huNoVs, particularly in studying virus-host interactions that may involve two host carbohydrate receptors or co-receptors for infection.

• Date created

  2015

• Subjects / Keywords

  • Virus-Host Interactions
  • Molecular Biology

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/R34J0B94Q

• License

  Attribution 4.0 International