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Skip to Search Results- 1Acedo, Jeella Z
- 1Bakhtiary, Alireza
- 1Cochrane, Rachel V K
- 1Engelhardt, Daniel
- 1Fernandez, Kleinberg Xiandrell
- 1Huang, Zedu
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Biochemical investigations of two polyketide synthase subclasses – highly reducing/non-reducing pairs, and polyketide synthase non-ribosomal peptide synthetases.
DownloadSpring 2016
Cladosporin is a polyketide metabolite that is biosynthesized by a highly reducing (HR)/non-reducing (NR) polyketide synthase (PKS) pair, wherein they catalyse head-totail condensation of acetyl and malonyl CoA units with subsequent β-keto modification. Cladosporin has recently been reported as a...
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Biosynthetic Study of CcsA and a Polyketide Synthase with Homology to Carnitine Acyltransferase
DownloadFall 2017
Polyketides are a class of natural products with large structural and biochemical diversity. Polyketides are assembled by the polymerization of short-chain fatty acids by enzymes named polyketide synthases. Fungal iterative polyketide synthases are notable in that they contain multiple active...
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Chemical Synthesis and Biological Evaluation of Unusual Sulfur-Containing Peptides and Efforts Toward Chemical Synthesis of Crocin
DownloadFall 2012
In order to minimize side reactions when preparing peptides containing a C-terminal cysteine residue, a method of protection of the carboxyl of the C-terminal cysteine as a cyclic ortho ester was developed. N-Fmoc-S-StBu cysteine cyclic ortho esters 31 (L) and 32 (D) were synthesized with...
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Chemical Synthesis to Understand and Influence Biological Systems: Spin Labels, Drug Development, and Biosynthetic Investigations
DownloadFall 2022
Spin labels are a useful tool towards the study of protein conformation and enable the mapping of interactions between specific positions within these systems. Synthetic routes towards three different chiral spin-labelled amino acids have been developed. Compared to commercially available spin...
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Development and Application of Protected Amino Acids for the Installation of Stable Post-translational Modifications Using Solid-Phase Peptide Synthesis
DownloadFall 2022
This thesis describes the synthesis of non-canonical amino acid building blocks and their use in solid-phase peptide synthesis (SPPS) to explore peptidic drug analogues based on natural products. We replaced sensitive functionalities found in the structure of naturally produced peptides with...
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Fall 2022
Peptides can elicit a whole host of beneficial physiological effects. The market for peptide-based therapeutics is rapidly expanding due to their extreme specificity and low toxicity. Conversely, they often possess poor bioavailability, stability, pharmacokinetics and cell permeability, limiting...
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Spring 2016
Peptides have a host of beneficial physiological effects and are often considered for therapeutic application to treat disease. However, poor bioavailabilities, problems with administration, sensitivity to environmental factors, and susceptibility to proteases limit them. The work presented in...
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Examining the structure, function and mode of action of bacteriocins from lactic acid bacteria
DownloadSpring 2010
Carnocyclin A (CclA) is a remarkably stable, potent bacteriocin produced by Carnobacterium maltaromaticum UAL307. Elucidation of the amino acid and genetic sequences revealed that CclA is a circular bacteriocin. Preliminary structural studies (dynamic light scattering, NMR, circular dichroism,...
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Functional Genomics of Bacterial and Fungal Biocontrol Agents for Biotechnological Applications
DownloadSpring 2022
Microbial-based biotechnologies were developed using combinations of functional genomics, biochemical tools, and a tripartite pine tree-beetle-fungal infection model. Several bacterial and fungal species were probed for natural product biosynthesis potential using whole genome sequencing while...
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Investigation of bioactive natural compounds for cardiovascular diseases and daqu fermentation, and the exploration of Beauveria bassiana as a biocontrol agent for the mountain pine beetle epidemic
DownloadSpring 2024
Fernandez, Kleinberg Xiandrell
Peptide-based compounds are crucial components of current research and development efforts, namely in the field of pharmaceutical therapeutics. Peptides possess complex secondary structures, resulting in a wide array of functions (e.g., signalling, or cellular structural capabilities). Advances...