Usage
  • 24 views
  • 18 downloads

Biochemical investigations of two polyketide synthase subclasses – highly reducing/non-reducing pairs, and polyketide synthase non-ribosomal peptide synthetases.

  • Author / Creator
    Cochrane, Rachel V K
  • Cladosporin is a polyketide metabolite that is biosynthesized by a highly reducing (HR)/non-reducing (NR) polyketide synthase (PKS) pair, wherein they catalyse head-totail condensation of acetyl and malonyl CoA units with subsequent β-keto modification. Cladosporin has recently been reported as a potent anti-malarial, acting as a nanomolar inhibitor of the Plasmodium falciparum lysyl tRNA synthetase. Sequencing of the genome of the producer organism, Cladosporium cladosporioides, allowed for identification of the cladosporin gene cluster. The HR and NR PKSs encoded therein, Cla2 and Cla3 respectively, were heterologously expressed. A series of substrate analogues were then synthesized and used in enzyme assays with Cla2. These studies showed that Cla2 could produce cladosporin from the advanced pentaketide intermediate. Cla2 also accepts analogues that contain the “unnatural” configuration at the key β- hydroxy group in the molecule, but does not recognize intermediates in which this β- hydroxy group is absent. Furthermore, Cla2 accepts analogues with shorter carbon chains, but not longer than is naturally present, suggesting a hydrophobic pocket of restricted size in the enzyme. The polyketide 10,11-dehydrocurvularin (DHC) is also biosynthesized by a HR/NR PKS pair. The genome of the producer organism, Alternaria cinerariae, was sequenced, and the DHC PKSs expressed heterologously in yeast. Availability of similar DHC-producing PKSs from Aspergillus terreus allowed us to compare biosynthetic machinery for the same metabolite in different organisms. Bioinformatic analyses suggest that sequence differences between the two sets of proteins are concentrated in specific domains, where they can be found mainly on the outer surface and specific faces of the protein. Lovastatin belongs to a group of therapeutics known as the statins, which are widely prescribed in patients with elevated levels of cholesterol. Lovastatin production is mediated by a HR PKS, LovB, in conjunction with an enoyl reductase partner, LovC, in Aspergillus terreus. During the biosynthesis of lovastatin, an interesting enzyme catalysed Diels-Alder reaction is proposed to occur in order to generate the correct stereochemistry of the decalin core. LovB contains an unusual truncated non-ribosomal peptide synthetase (NRPS) portion at its C terminus, which includes a condensation (C) domain that is thought to catalyse this reaction. In order to test this hypothesis, a series of linear hexaketide analogues, which are unable to undergo cyclization, were synthesized. These intermediates were sent to our collaborators for co-crystallization with the LovB CON domain. A 13C labeled tetraketide intermediate was also synthesized for feeding studies with LovB, to confirm its intermediacy in the biosynthesis. Chaetoglobosin A and cytochalasin E belong to a group of polyketides collectively known as the cytochalasins. This group of molecules is made by PKS-NRPS enzymes, which synthesize the polyketide backbone using their PKS domains and then add an amino acid using the NRPS portion. The polyketide-amino acid intermediate is proposed to undergo reductive release from the enzyme, followed by cyclizations and oxidation to afford the final metabolites. The exact process by which these post-PKS modifications occur is still unknown. With hopes of studying these mechanisms in more detail, synthesis of an advanced polyketide intermediate was initiated. Further elaboration of the synthesized compounds will afford the mature polyketide chain which will be sent to our collaborators in UCLA for enzymatic assays with the respective PKS-NRPS.

  • Subjects / Keywords
  • Graduation date
    2016-06
  • Type of Item
    Thesis
  • Degree
    Doctor of Philosophy
  • DOI
    https://doi.org/10.7939/R3RB6WC7G
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Doctoral
  • Department
    • Department of Chemistry
  • Supervisor / co-supervisor and their department(s)
    • Vederas, John (Chemistry)
  • Examining committee members and their departments
    • Li, Liang (Chemistry)
    • Campbell, Robert (Chemistry)
    • Gerwick, William (Skaggs School of Pharmacy and Pharmaceutical Sciences)
    • Cairo, Christopher (Chemistry)