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- 227Chemistry, Department of/Journal Articles (Chemistry)
- 12Chemistry, Department of/Research Data and Materials (Chemistry)
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- 69Klassen, John S.
- 54Serpe, Michael J.
- 52Kitova, Elena N.
- 18Michaelis, V.K.
- 17Gao, Yongfeng
- 14Loppnow, Glen R.
- 20Electrospray ionization mass spectrometry
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Reliable determinations of protein-ligand interactions by direct ESI-MS measurements: Are we there yet?
Download2012
El-Hawiet, Amr, Klassen, John S., Kitova, Elena N., Schnier, Paul D.
The association-dissociation of noncovalent interactions between protein and ligands, such as other proteins, carbohydrates, lipids, DNA, or small molecules, are critical events in many biological processes. The discovery and characterization of these interactions is essential to a complete...
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Reliable determinations of protein-ligand interactions by direct ESI-MS measurements: Are we there yet?
Download2012
Klassen, John S., Kitova, Elena N., Schnier, Paul D., El-Hawiet, Amr
The association-dissociation of noncovalent interactions between protein and ligands, such as other proteins, carbohydrates, lipids, DNA, or small molecules, are critical events in many biological processes. The discovery and characterization of these interactions is essential to a complete...
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Resonance Raman Intensities Demonstrate that C5 Substituents Affect the Initial Excited-state Structural Dynamics of Uracil More than C6 Substituents
Download2016-01-01
Teimoory, Faranak, Loppnow, Glen R.
Resonance Raman derived initial excited‐state structural dynamics provide insight into the photochemical mechanisms of pyrimidine nucleobases, in which the photochemistry appears to be dictated by the C5 and C6 substituents. The absorption and resonance Raman spectra and excitation profiles of...
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2015-01-01
Li, Xue, Gao, Yongfeng, Serpe, Michael J.
Poly (N‐isopropylacrylamide) (pNIPAm)‐based hydrogels and hydrogel particles (microgels) have been extensively studied since their discovery and “popularization” a few decades ago. While their uses seem to have no bounds, this Feature Article is focused on their development and application for...
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2013-01-01
Islam, Molla R., Lu, Zhenzhen, Li, Xue, Sarker, Avijeet K., Hu, Liang, Choi, Paul, Li, Xi, Hakobyan, Narek, Serpe, Michael J.
Stimuli-responsive polymers are capable of translating changes in their local environment to changes in their chemical and/or physical properties. This ability allows stimuli-responsive polymers to be used for a wide range of applications. In this review, we highlight the analytical applications...
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2017-01-01
Li, Xue, Cai, Xiangbin, Gao, Yongfeng, Serpe, Michael J.
Temperature and pH responsive semi-interpenetrating network (semi-IPN) hydrogel-based bilayer actuators were fabricated by generating a poly(N-isopropylacrylamide) (pNIPAm)-based hydrogel in the presence of positively charged polyelectrolyte poly(diallyldimethylammonium chloride) (pDADMAC) on a...
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Screening anti-cancer drugs against tubulin using catch-and-release electrospray ionization mass spectrometry
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Kitova, Elena N., Klassen, John S., Winter, Philip, Tuszynski, Jack A., Darestani, Reza R.
Tubulin, which is the building block of microtubules, plays an important role in cell division. This critical role makes tubulin an attractive target for the development of chemotherapeutic drugs to treat cancer. Currently, there is no general binding assay for tubulin–drug interactions. The...
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Screening anti-cancer drugs against tubulin using catch-and-release electrospray ionization mass spectrometry
Download2016
Kitova, Elena N., Klassen, John S., Winter, Philip, Tuszynski, Jack A., Darestani, Reza R.
Tubulin, which is the building block of microtubules, plays an important role in cell division. This critical role makes tubulin an attractive target for the development of chemotherapeutic drugs to treat cancer. Currently, there is no general binding assay for tubulin–drug interactions. The...
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Screening carbohydrate libraries for protein interactions using the direct ESI-MS assay: Applications to libraries of unknown concentration
Download2014
El-Hawiet, Amr, Klassen, John S., Kitova, Elena N.
A semiquantitative electrospray ionization mass spectrometry (ESI-MS) binding assay suitable for analyzing mixtures of oligosaccharides, at unknown concentrations, for interactions with target proteins is described. The assay relies on the differences in the ratio of the relative abundances of...
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Screening carbohydrate libraries for protein interactions using the direct ESI-MS assay: Applications to libraries of unknown concentration
Download2014
El-Hawiet, Amr, Klassen, John S., Kitova, Elena N.
A semiquantitative electrospray ionization mass spectrometry (ESI-MS) binding assay suitable for analyzing mixtures of oligosaccharides, at unknown concentrations, for interactions with target proteins is described. The assay relies on the differences in the ratio of the relative abundances of...