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Skip to Search Results- 69Klassen, John S.
- 54Serpe, Michael J.
- 52Kitova, Elena N.
- 18Michaelis, V.K.
- 17Gao, Yongfeng
- 14Loppnow, Glen R.
- 20Electrospray ionization mass spectrometry
- 14Etalons
- 13SSNMR
- 12Affinity
- 12Stimuli-Responsive Polymers
- 11Complexes
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2014
Ahiabu, Andrews, Islam, Molla R., Li, Xue, Serpe, Michael J.
Responsive polymer-based materials have found numerous applications due to their ease of synthesis and the variety of stimuli that they can be made responsive to. In this review, we highlight the group’s efforts utilizing thermoresponsive poly (N-isopropylacrylamide) (pNIPAm) microgel-based...
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2016-01-01
Jiang, Yaxin, Colazo, Marcos G., Serpe, Michael J.
Progesterone (P4) is a vital steroid hormone involved in the reproduction cycle and necessary for maintenance of pregnancy in humans and animals. Monitoring P4 concentrations is required in various fields, such as dairy industry, environmental protection, and clinical laboratories. The current...
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Poly (N-isopropylacrylamide)-co-(acrylic acid) microgel/ag nanoparticle hybrids for the colorimetric sensing of H2O2
Download2015
Zhang, Qiang M., Serpe, Michael J., Han, De-Man
Poly (N-isopropylacrylamide)-co-(acrylic acid) (pNIPAm-co-AAc) microgels composed of Ag nanoparticles (Ag NPs) have been synthesized and employed for the colorimetric sensing of H2O2. Each pNIPAmco-AAc microgel, which exhibited a diameter of ∼800 nm, contained multiple Ag NPs (diameter of ∼5 nm),...
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2014
Serpe, Michael J., Islam, Molla R.
In this submission we fabricated a humidity-responsive polymer-based actuator by layering negatively charged poly(N-isopropylacrylamide)-co-acrylic acid microgels and positively charged poly(diallyldimethyl ammonium chloride) on top of a flexible plastic substrate. We show that the extent of the...
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Polymer-based devices for the label-free detection of DNA in solution: low DNA concentrations yield large signals
Download2014-01-01
Islam, Molla R., Serpe, Michael J.
Poly (N-isopropylacrylamide-co-N-(3-aminopropyl) methacrylamide hydrochloride) microgel-based optical devices (etalons) have been shown to change their optical properties in the presence of single-stranded DNA. We hypothesize that this is due to the negatively charged DNA penetrating through the...
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2017-01-01
Carvalho, Wildemar S. P., Wei, Menglian, Ikpo, Nduka, Gao, Yongfeng, Serpe, Michael J.
Reports in the literature on the development of new chemical sensing technologies have been growing steadily in number for years. For example, a Web of Science topic search using the term “Chemical Sensing” revealed that over 8 000 papers were published in 2015–2017, with over 40 000 citations to...
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2018-01-01
El-Yazbi, Amira F., Loppnow, Glen R.
Hepatocellular carcinoma is one of the most common malignant tumors in the world. Chronic hepatitis B and C infections are the most common etiologies of hepatocellular carcinoma worldwide. In this study, we explore the potential DNA damaging effect of some FDA-approved antiviral drugs which may...
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Protein-glycolipid interactions studied in vitro using ESI-MS and nanodiscs: Insights into the mechanisms and energetics of binding
Download2015
Han, Ling Han, Kitova, Elena N., Klassen, John S., Pluvinage, Benjamin, Lin, Hong, Nikjah, Sanaz, Li, Jun, Boraston, Alisdair B.
Electrospray ionization-mass spectrometry (ESI-MS) analysis combined with the use of nanodiscs (NDs) to solubilize glycolipids (GLs) has recently emerged as a promising analytical method for detecting protein–GL interactions in vitro and, when applied to libraries of GLs, ranking their...
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Protein-glycolipid interactions studied in vitro using ESI-MS and nanodiscs: Insights into the mechanisms and energetics of binding
Download2015
Nikjah, Sanaz, Boraston, Alisdair B., Lin, Hong, Kitova, Elena N., Li, Jun, Pluvinage, Benjamin, Han, Ling Han, Klassen, John S.
Electrospray ionization-mass spectrometry (ESI-MS) analysis combined with the use of nanodiscs (NDs) to solubilize glycolipids (GLs) has recently emerged as a promising analytical method for detecting protein–GL interactions in vitro and, when applied to libraries of GLs, ranking their...
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Quantifying labile protein-ligand interactions using electrospray ionization mass spectrometry
Download2010
El-Hawiet, Amr, Klassen, John S., Kitova, Elena N., Liu, Lan
A new electrospray ionization mass spectrometry (ES-MS) approach for quantifying protein—ligand complexes that are prone to in-source (gas-phase) dissociation is described. The method, referred to here as the reference ligand ES-MS method, is based on the direct ES-MS assay and competitive ligand...