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Fall 2014

Sajadfar,Narges

To address the requirement of dynamic pricing and cost control in high-variation product manufacturing, nowadays many companies face the problem of generating quotes and order prices timely, accurately and consistently. This research reports a preliminary investigation on automatic cost estimation

address effective and efficient product cost structures, frequent configuration variations and business changes. Cost feature has been defined by the authors as a unique class in the unified feature modelling system to address the characteristics of cost engineering entities, constraints and dependency

relations. This research describes the relations between a cost feature and three engineering sub-models, i.e. machining model, design model and other auxiliary data model by associating tangible and intangible data. Further, semantic relations are investigated in early product design process for dynamic

Fall 2021

Campbell, Hazel V

Many software engineering problems have traditionally been approached by applying techniques based on static analysis and fixed sets of rules. I created two novel techniques to tackle three software engineering problems: typo location, fix suggestion, and crash report bucket creation. However

) software crash reports. In all cases, performance (in terms of F1-score) matched or beat commonly used rule-based techniques. The TF-IDF-driven approach can adapt automatically to patterns in crash reports as they evolve. Additionally, several side benefits arose from using statistical techniques.Some

Fall 2015

Lai, Yan Tiffany

ubiquitin modifier 1 (SUMO-1) in pancreatic islet cells. The creation of these plasmids is a proof of the versatility and modularity of the ddFP and its related FP exchange (FPX) biosensor design strategy. We also report the engineering of a dark “A” copy (DA) for ddFP applications. By a simple rational

The expansion of the fluorescent protein (FP) colour palette through discovery of more species possessing FPs and by subjecting them to intensive protein engineering efforts has greatly aided the effectiveness of FP-based biosensor designs. Currently, the main FP-based methods for detecting protein

may facilitate multicolour imaging of biosensors in live cells due to the fact that they have a narrower spectral profile than a FRET pair. In this thesis, we describe our efforts to expand the range of applications of ddFP in live cells. We report the creation of systems for the sensing of global O

Spring 2022

Bilotta, Miguelangel Jose

Slender, masonry loadbearing walls made with concrete blocks are one of the most frequently used masonry structural systems in North America. They are commonly found in commercial and industrial single-storey construction, such as warehouses, and in school gymnasiums, auditoriums, and retail

2008

Crick, David A. (David Andrew), 1965-245 10

Spring 2020

Alyenbaawi, Hadeel E

to understand the prion-like mechanism of the pathology of tau protein spreading in TBI patients by establishing a novel TBI paradigm to use in a zebrafish larvae model, as well as engineering a novel tau protein biosensor in transparent zebrafish that can be used to report tau seeding and spreading

in vivo. We successfully isolated a stable transgenic zebrafish line that expresses a tauopathy biosensor reporter protein and validated that our transgenic zebrafish can report various forms of tau seeds via intraventricular injections. The biosensor output reports tau aggregation as GFP+ve puncta

significantly reduced tau protein aggregation in our TBI model, hence demonstrating the important role of dynamin in the uptake of tau seeds and spreading of tau protein in TBI. The engineering of our novel tau protein biosensor and establishment of the TBI model in larval zebrafish will not only uncover more

Fall 2014

Zhao, Yongxin

-based indicators remains a challenging engineering problem, mainly due to lack of structural information for rational design and effective methodologies of protein engineering. The goal of this thesis work is to tackle the long-standing challenge of engineering FP-based indicators for improved

engineering of single FP-based Ca2+ indicator and led to several variants with improved performance and various new colors. This palette of new Ca2+ indicators enables simultaneous monitoring of Ca2+ transients in different cellular compartments or different types of cells, which opens up a new era of

Spring 2018

Han, Linbo

and surface engineering. We report a novel and universal mussel-inspired anchoring strategy based on cationic amine-modified catechol ligand coupled with 2-methacryloyloxyethyl phosphorylcholine (MPC) moiety. The ligand shows substrate-independent anchoring capability, and the deposited film possesses

monitoring (QCM-D). Adsorption and desorption of biomolecules on/from polyelectrolyte surfaces play a critical role in numerous biomedical and engineering applications. Though some weak polyelectrolytes have been developed for protein adsorption and desorption by regulating the salinity and pH, limited

reports are available about the regeneration of strong polyelectrolyte surfaces between protein-attractive and protein-repulsive states, which is highly desirable but challenging to realize as it is more difficult to fully release the pre-adsorbed proteins from strong polyelectrolytes due to their

Fall 2017

Zhang, Wei

proteins have required some degree of protein engineering for improved optical and functional properties. Encouragingly, an expanded range of optogenetic tools with novel functions has been developed by coupling the natural photosensory mechanisms with certain proteins. Through such efforts, the

optogenetic toolkit is rapidly growing, though it is still relatively limited. In this thesis, we describe our efforts to expand the scope of photosensory mechanisms by engineering a photocleavable protein (PhoCl) that splits into two fragments upon violet light (~400 nm) illumination. PhoCl is a

-forming glycoprotein, Pannexin-1. We also report the development, optimization, and characterization of the first genetically encoded near infrared (NIR) fluorescent pH indicator, pH-mIFP. The pH dependent fluorescence was introduced to the NIR FP by rational design, which was followed by

Spring 2016

Jamieson, Marnie V.

enhance the interactions between instructors and student design teams in the face of increasing enrollment and to align the course strategically with attributes expected for graduating engineers set out by the University and elaborated in the Canadian Engineering Accreditation Board (CEAB) Guidelines

engagement and satisfaction, and will include post course interview and survey data. This thesis contributes to the development of a continuous improvement process for teaching and learning for Chemical Engineering students, by creating a blended course design based on constructive alignment with program

objectives, learning activities and performance based assessment consistent with the Canadian Engineering Accreditation Board Graduate Attribute Assessment. Examination of factors that impact the quality of the learning experience for students and instructors, studying the impacts of changes, tool