Search
Skip to Search Results- 33Mass-spectrometry
- 24Chemistry
- 23Organic compounds. Synthesis.
- 22Metabolomics
- 21Capillary electrophoresis.
- 21Catalysis
-
Development and Application of Chemical Isotope Labeling Methods and Metabolite Identification Solution for Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadFall 2018
Metabolomics, the comprehensive analysis of small molecules in biological specimens, has become an emerging and indispensable tool for systems biology and clinical research. Liquid chromatography-mass spectrometry (LC-MS) is a dominant analytical platform for metabolomics, featuring high...
-
Development and Application of Chemical Isotope Labeling Methods for Metabolomics Data Processing and Liquid Chromatography-Mass Spectrometry-Based Metabolomics
DownloadSpring 2016
Metabolomics is the study of chemical processes involving small-molecule metabolites in a given biological system. These small molecules are downstream outputs of cellular activities. An understanding of the metabolome change can help us to visualize perturbations in the genome or proteome from...
-
Development and Application of ESI-MS Based Techniques for Quantitative Determination of Protein-Carbohydrate Interactions
DownloadFall 2016
This thesis describes the development and application of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was employed to quantify protein-carbohydrate interactions in aqueous buffer...
-
Development and Application of ESI-MS Based Techniques to Study Non-Covalent Protein-Ligand Complexes in Solution and the Gas Phase
DownloadSpring 2013
This thesis describes the development and application of electrospray ionization mass spectrometry (ESI-MS) based techniques to study noncovalent protein-ligand complexes in solution and the gas phase. The application of the direct ESI-MS assay for quantifying the stoichiometry and absolute...
-
Development and Application of Isotope Labeling Method and Data Processing Program for Liquid Chromatography Mass Spectrometry Based Metabolome Profiling
DownloadSpring 2015
The objective of this thesis focuses on establishing a software tool (IsoMS) used in the quantification and qualification of metabolites in complex biological samples, developing robust methods to extract metabolites from different plant samples, and applying a differential 13C-/12C-isotope...
-
Development and application of mass spectrometry for bioanalysis of proteins and metabolites
DownloadSpring 2011
The proteome, a hybrid term from protein and genome, is the total set of proteins expressed by a genome in a cell, tissue, or organism at a given time. Proteomics, the study of the proteome, is concerned with a variety of topics such as identity, quantity, activity, structure, protein-protein...