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2018-01-01
Shoute, Lian C. T., Loppnow, Glen R.
Understanding the dsDNA·EG binding interaction is important because the EvaGreen (EG) dye is increasingly used in real-time quantitative polymerase chain reaction, high resolution melting analysis, and routine quantification of DNA. In this work, a binding isotherm for the interactions of EG with...
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Probing microscopic conformational dynamics in folding reactions by measuring transition paths
Download2019-12-01
Hoffer, Noel Q, Woodside, Michael T
Transition paths comprise those parts of a folding trajectory where the molecule passes through the high-energy transition states separating folded and unfolded conformations. The transition states determine the folding kinetics and mechanism but are difficult to observe because of their brief...