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Exploiting bacterial glycosylation machineries for the synthesis of a Lewis antigen-containing glycoprotein
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Hug, Isabelle, Reiz, Bela, Fentabil, Messele A., Klassen, John S., Whittal, Randy M., Zheng, Blake, Feldman, Mario F.
Glycoproteins constitute a class of compounds of increasing importance for pharmaceutical applications. The manipulation of bacterial protein glycosylation systems from Gram-negative bacteria for the synthesis of recombinant glycoproteins is a promising alternative to the current production...
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Fall 2019
This thesis focuses on the development and application of electrospray ionization mass spectrometry (ESI-MS) based methods for characterizing glycoproteins (GPs) and investigating their interactions with glycan binding proteins (GBPs) and drugs. A multipronged approach was developed to identify...