Search
Skip to Search Results- 1Alexander, Anthony John.
- 1Chen, Lu
- 1Curle, Jared P
- 1Dawe, Margot Renee
- 1De Souza, Andrea
- 1Dunlop, Kevin Yoshio.
-
Fall 2017
The most often used biofluids in metabolomics and disease biomarker discovery studies in the literature include urine, processed blood (serum or plasma), and cerebral spinal fluid. While investigations using these biofluids have led to numerous innovations in the field of metabolomics, little...
-
Differential 15N2-/14N2-isotope Dansylhydrazine Labeling and LC-MS for Quantification of the Human Carbonyl Metabolome
DownloadFall 2011
The objective of this work was the design and use of paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust means of quantitative mass spectrometry (MS) based metabolome profiling. Herein is presented the differential 15N2-/14N2-isotope...
-
Evaluation of Derivatization Reaction Conditions for Metabolite Labelling Using Differential Isotopic Labelling for Biological Samples Analyzed by Liquid Chromatography Mass Spectrometry
DownloadFall 2013
The objective of this work was to assess the impact of reaction conditions for isotopic labeling of metabolites prior to analysis using liquid chromatography mass spectrometry (LC-MS). Differential isotope 12C/13C dansyl chloride was used to label metabolites containing either primary amines,...
-
Investigating Protein-Carbohydrate Interactions with Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS)
DownloadFall 2014
The application of hydrogen/deuterium exchange mass spectrometry (HDX-MS) to investigating protein-carbohydrate interactions is described. Proteins from three bacterial toxins, the B subunit homopentamers of Cholera toxin (CTB5) and Shiga toxin type 1 (Stx1B5) and a fragment of Clostridium...
-
Isotope Labeling Liquid Chromatography Mass Spectrometry: Metabolite Identification and Human Salivary Metabolome Profiling
DownloadFall 2012
The objective of this work was to use paired labeling reagents that are chemically identical but isotopically different to provide a simple and robust way of mass spectrometry (MS)-based metabolome profiling. A differential 13C-/12C-isotope dansyl chloride (DnsCl) derivatization strategy has been...
-
Metabolite Identification and Quantification in Biofluids Using Liquid Chromatography Mass Spectrometry
DownloadFall 2012
Metabolomics, one of the branches of systems biology, is the comprehensive measurement of all the endogenous metabolites in a biological system. It can be applied in the areas of biomarker discovery and diagnosis of critical diseases and disorders, and can significantly increase our understanding...
-
Metabolomics by GC×GC-TOFMS: Overcoming Challenges from Sample Preparation to Data Analysis Towards Standardization of the Workflow
DownloadSpring 2021
Metabolomics is an ‘omics’ field, which involves the comprehensive characterization of metabolites using analytical chemistry technologies and statistical methods to interpret the results. The term metabolomics was coined about two decades ago as an analogy to other precedent omics approaches:...
-
Spring 2022
Metabolomics, as an interdisciplinary research area, requires the integration of knowledge from different disciplines, such as analytical chemistry, bioinformatics, and statistics. The general workflow of metabolome analysis includes sample preparation, data acquisition, data processing, data...