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Skip to Search Results- 10Gänzle, Michael G.
- 3Garcia-Hernandez, Rigoberto
- 3McMullen, Lynn M.
- 2Li, Hui
- 2Mercer, Ryan G.
- 2Yang, Xianqin
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Comparative assessment of qPCR enumeration methods that discriminate between live and dead Escherichia coli O157:H7 on beef
Download2018-01-01
Laidlaw, Anna M., Gänzle, Michael G., Yang, Xianqin
Quantitative Polymerase Chain Reaction (qPCR) is a molecular method commonly used to detect and quantify bacterial DNA on food but is limited by its inability to distinguish between live and dead cell DNA. To overcome this obstacle, propidium monoazide (PMA) alone or with deoxycholate (DC) was...
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Development and validation of a surrogate strain cocktail to evaluate bactericidal effects of pressure on verotoxigenic Escherichia coli
Download2015-01-01
Garcia-Hernandez, Rigoberto, McMullen, Lynn, Gänzle, Michael G.
Many strains of verotoxigenic Escherichia coli (VTEC) are highly resistant to pressure. To facilitate future studies to improve the elimination of VTEC by pressure processing of food, this study developed and validated a cocktail of non-pathogenic strains of E. coli with equal or higher...
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Effect of chitosan, and bacteriocin – Producing Carnobacterium maltaromaticum on survival of Escherichia coli and Salmonella Typhimurium on beef
Download2018-01-01
Hu, Zi Yi, Balay, Danielle, Hu, Ying, McMullen, Lynn M., Gänzle, Michael G.
The aim of this study was to investigate the synergistic effect of chitosan and bacteriocins against Escherichia coli and Salmonella in media and in lean beef. The inhibitory effects of chitosan and bacteriocins against E. coli AW1.7 and S. enterica Typhimurium in media were determined by a...
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Effect of hydrostatic pressure and antimicrobial on survival of Listeria monocytogenes and enterohaemorrhagic E.coli in beef
Download2016-01-01
The effects of marinades on Listeria monocytogenes and enterohaemorrhagic Escherichia coli were investigated in pressure treated beef steaks. Meat was treated with 600 MPa or 450 MPa. Marinades did not enhance pressure inactivation of E. coli in beef steaks and marinades prevented...
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2016-01-01
Li, Hui, Garcia-Hernandez, Rigoberto, Driedger, Darcy, McMullen, Lynn M., Gänzle, Michael G.
The pressure resistance of Shiga-toxin producing Escherichia coli (STEC) depends on food matrix. This study compared the resistance of two five-strain E. coli cocktails, as well as the pressure resistant strain E. coli AW1.7, to hydrostatic pressure application in bruschetta, tzatziki, yoghurt...
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2015
Garcia-Hernandez, Rigoberto, Ruan, Lifang, Gänzle, Michael G., Zheng, Jinshui, Mercer, Ryan G., McMullen, Lynn M.
Escherichia coli AW1.7 is a heat resistant food isolate and the occurrence of pathogenic strains with comparable heat resistance may pose a risk to food safety. To identify the genetic determinants of heat resistance, 29 strains of E. coli that differed in their of heat resistance were analyzed...
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Improving beef hamburger quality and fatty acid profiles through dietary manipulation and exploitation of fat depot heterogeneity
Download2014
Dugan, Michael E. R., Rolland, David C., Uttaro, Bethany, Aalhus, Jennifer L., Block, Hushton C., Vahmani, Payam, Mapiye, Cletos, Proctor, Spencer D., McAllister, Timothy A.
Background Hamburger is the most consumed beef product in North America, but lacks in nutritional appeal due to its high fat content and high proportion of saturated fatty acids (SFA). Objectives of the present study were to improve the FA profiles of hamburgers made with perirenal fat (PRF) and...
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Influence of cyclopropane fatty acids on heat, high pressure, acid, and oxidative stress resistance in Escherichia coli
Download2016-01-01
Chen, Yuan Yao, Gänzle, Michael G.
Heat and high pressure resistant strains of Escherichia coli are a challenge to food safety. This study investigated effects of cyclopropane fatty acids (CFAs) on stress tolerance in the heat- and pressure-resistant strain E. coli AW1.7 and the sensitive strain E. coli MG1655. The role of CFAs...
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Initial differences in lipid processing leading to pig- and beef-derived mature adipocyte dedifferentiation
Download2009
Fernyhough, Melinda E., Hausman, Gary J., Guan, Leluo, Guridi, Maitea, Jiang, Zhihua, Dodson, Michael V., Chen, Jie
Clonal cultures of pig-derived mature adipocytes are capable of dedifferentiating and forming proliferative-competent progeny cells in vitro. Initial lipid processing, is different to that observed in cultures of beef-derived adipocytes. Mature pig adipocytes extrude lipid before proliferation,...
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Mechanisms of pressure-mediated cell death and injury in Escherichia coli: From fundamentals to food applications
Download2015
High hydrostatic pressure is commercially applied to extend the shelf life of foods, and to improve food safety. Current applications operate at ambient temperature and 600 MPa or less. However, bacteria that may resist this pressure level include the pathogens Staphylococcus aureus and strains...