Initial differences in lipid processing leading to pig- and beef-derived mature adipocyte dedifferentiation

• Author(s) / Creator(s)

  • Fernyhough, Melinda E.
  • Hausman, Gary J.
  • Guan, Leluo
  • Guridi, Maitea
  • Jiang, Zhihua
  • Dodson, Michael V.
  • Chen, Jie

• Clonal cultures of pig-derived mature adipocytes are capable of dedifferentiating and forming proliferative-competent progeny cells in vitro. Initial lipid processing, is different to that observed in cultures of beef-derived adipocytes. Mature pig adipocytes extrude lipid before proliferation, whereas beef-derived adipocytes symmetrically, or asymmetrically, divide without expelling lipid. These observations suggest that dedifferentiation of mature adipocytes relies on species-specific mechanisms, or that different culture conditions are required for pigderived adipocytes to dedifferentiate in a manner similar to beef adipocytes. This in vitro system will aid in our understanding of lipid metabolism, regulation of single cells, processes involved in dedifferentiation of cells, and/or characteristics of putative stem cells residing in adipose tissue.

• Date created

  2009

• Subjects / Keywords

  • Lipid Extrusion
  • Adipocyte
  • Dedifferentiation
  • Beef
  • Pig

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/R3ZS2KS7Z

• License

  Attribution-NonCommercial 4.0 International