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Fall 2010
In this thesis, I characterized the association of Arf4•GDP with ER-Golgi intermediate compartment membranes. We confirmed that GDP-arrested Arf4 mutants associated with membranes irrespective of nature of tag or mutation. Recruitment appeared specific since loss of N-terminal myristoylation...
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Characterization of GBF1, Arfs and COPI at the ER-Golgi intermediate compartment and mitotic Golgi clusters
DownloadFall 2009
Protein trafficking between the endoplasmic reticulum (ER) and Golgi complex is regulated by the activity of ADP-ribosylation factors (Arfs). Arf activation by guanine nucleotide exchange factors (GEFs) leads to the recruitment of the coatomer protein COPI and vesicle formation. By using...