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- 24Chemistry
- 23Organic compounds. Synthesis.
- 22Metabolomics
- 21Capillary electrophoresis.
- 21Catalysis
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Development and Characterization of Interfacial Chemistry for Biomolecule Immobilization in Surface Plasmon Resonance (SPR) Imaging Studies
DownloadFall 2009
Surface immobilization of probe molecules in surface based assays is a key area of research in the continued development of immunoassay microarrays. Interest continues to grow in microarray based immunoassays given their potential as a high throughput technique for immunodiagnostics. Therefore,...
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Development and Characterization of New Stationary Phases for Hydrophilic Interaction Liquid Chromatography
DownloadSpring 2014
Ibrahim, Mohammed Elsayed Abdelatif
Hydrophilic interaction liquid chromatography (HILIC) has gained high popularity among separation scientists in the last two decades due to its ability to retain polar analytes. Many new HILIC stationary phases with different selectivities have appeared in the recent literature. Such developments...
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Development and optimization of a high through-put screening methodology for rapid dynamic range improvement of FRET-based biosensors
DownloadSpring 2012
Abdel Latif Ibraheem, Ahmed Abdel Mohsen
Fluorescent protein (FP)-based biosensors based on the principle of intramolecular Förster resonance energy transfer (FRET) enable the visualization of a variety of biochemical events in living cells. The sensitivity of such biosensors is proportional to the change in ratiometric emission, and so...
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Development of a Binding-induced DNA Nanosensor Based on Fluorescence Resonance Energy Transfer
DownloadSpring 2013
This thesis describes a binding-induced DNA nanosensor and its application to homogeneous detection of DNA and proteins. This nanosensor was based on the binding-induced assembly of fluorescently labeled DNA on the DNA-functionalized quantum dots, resulting in fluorescence resonance energy...
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Fall 2012
The overall goal of this thesis research is to develop a spectral searching strategy capable of identifying peptide sequences from MS/MS spectra with high sensitivity and accuracy. First, a shotgun proteome analysis method was developed and successfully applied to the identification of proteins...