Development of a Binding-induced DNA Nanosensor Based on Fluorescence Resonance Energy Transfer

• Author / Creator

  Li, Xukun

• This thesis describes a binding-induced DNA nanosensor and its application to homogeneous detection of DNA and proteins. This nanosensor was based on the binding-induced assembly of fluorescently labeled DNA on the DNA-functionalized quantum dots, resulting in fluorescence resonance energy transfer (FRET) between the quantum dots (605 QDs) and the fluorescent dye (Cy5). The sensor was applied to the detection of single-nucleotide polymorphism (SNP) in the p53 gene sequence and the detection of platelet derived growth factor, with pM detection limits. This strategy can be applied to developing assays for a wide range of targets by functionalizing the surface of quantum dots with appropriate affinity ligands (e.g., aptamers and antibodies).

• Subjects / Keywords

  • Nanosensor
  • Fluorescence Resonance Energy Transfer
  • Binding-induced DNA assembly

• Graduation date

  Spring 2013

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3638S

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.