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- 6Proteomics
- 3Electrospray ionization
- 2Metabolomics
- 2Protein-carbohydrate interactions
- 2Spectral Library
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Spring 2016
The simultaneous quantification of all biological molecules, from metabolites to proteins, holds the key to discovering molecular signatures that can diagnose diseases before irreversible damages have occurred in the body. Recent advances in the field of liquid chromatography coupled mass...
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Development and Application of ESI-MS Based Techniques for Quantitative Determination of Protein-Carbohydrate Interactions
DownloadFall 2016
This thesis describes the development and application of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was employed to quantify protein-carbohydrate interactions in aqueous buffer...
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Development and application of mass spectrometry for bioanalysis of proteins and metabolites
DownloadSpring 2011
The proteome, a hybrid term from protein and genome, is the total set of proteins expressed by a genome in a cell, tissue, or organism at a given time. Proteomics, the study of the proteome, is concerned with a variety of topics such as identity, quantity, activity, structure, protein-protein...
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Development and Applications of Mass Spectrometric Methods for Proteome Analysis and Protein Sequence Characterization
DownloadFall 2013
Mass spectrometry (MS) is widely used in proteomic work for protein identification and characterization. A key to the success of MS for protein analysis is related to sample preparation. Current sample preparation methods do not meet all the needs of protein or proteome analysis. My thesis work...
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Development and Applications of Stable Isotope Labelling Liquid Chromatography Mass Spectrometry for Quantitative Proteomics
DownloadSpring 2011
This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...
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Fall 2012
The overall goal of this thesis research is to develop a spectral searching strategy capable of identifying peptide sequences from MS/MS spectra with high sensitivity and accuracy. First, a shotgun proteome analysis method was developed and successfully applied to the identification of proteins...
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Spring 2010
Interest in microfluidic platforms has surged as an alternative for sample preparation in the past two decades, with the potential for miniaturization, portability, automation, integration and parallelism driving this research. However, it is still very challenging to develop an integrated...
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Quantitative Characterization of Noncovalent Protein-Carbohydrate Interactions using Electrospray Ionization Mass Spectrometry
DownloadFall 2015
The interactions between water-soluble proteins and carbohydrates found on the surfaces of cells play important roles in many physiological and pathological cellular processes. Carbohydrates function as receptors for signaling, cellular recognition and adhesion, and pathogen infections. This...
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Separation and characterization of polycyclic aromatic hydrocarbons and azaarenes using normal phase liquid chromatography and electrospray ionization mass spectrometry
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Normal phase liquid chromatography (NPLC) is widely used for separating petroleum into compound classes, such as polycyclic aromatic hydrocarbons (PAHs) and polar nitrogen containing compounds. The charge-transfer and hypercrosslinked polystyrene NPLC phases provide unique selectivities for PAHs....