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- 11Chemistry, Department of/Journal Articles (Chemistry)
- 5Graduate and Postdoctoral Studies (GPS), Faculty of
- 5Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 1Biological Sciences, Department of
- 1Biological Sciences, Department of/Journal Articles (Biological Sciences)
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2016
Richards, Michele R., Campuzano, Iain D. G., Kitova, Elena N., Klassen, John S., Li, Jun, Privé, Gilbert G., Xiong, Zi Jian, Bagal, Dhanashri
Saposin A (SapA) lipoprotein discs, also known as picodiscs (PDs), represent an attractive method to solubilize glycolipids for protein interaction studies in aqueous solution. Recent electrospray ionization mass spectrometry (ESI-MS) data suggest that the size and composition of...
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2016
Klassen, John S., Campuzano, Iain D. G., Bagal, Dhanashri, Privé, Gilbert G., Kitova, Elena N., Xiong, Zi Jian, Richards, Michele R., Li, Jun
Saposin A (SapA) lipoprotein discs, also known as picodiscs (PDs), represent an attractive method to solubilize glycolipids for protein interaction studies in aqueous solution. Recent electrospray ionization mass spectrometry (ESI-MS) data suggest that the size and composition of...
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Spring 2013
Chavali Venkata Subramanya, Ravi Shankar
The In-gel polymerase chain reaction (PCR) technology developed by our group at the University of Alberta makes use of PCR reagents entrapped within nano-liter polyacrylamide gels. To adapt this technology for use at the point of care, methods were developed to preserve gel-based PCR reagents by...
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Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method
Download2009
Kitova, Elena N., Sun, Jiangxiao, Sun, Nian, Klassen, John S.
The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes...
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Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method
Download2009
Sun, Nian, Klassen, John S., Kitova, Elena N., Sun, Jiangxiao
The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes...
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Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method
2009
Kitova, Elena N., Klassen, John S., Sun, Nian, Sun, Jiangxiao
The application of the reporter molecule (Mrep) method for identifying nonspecific complexes in the ES-MS analysis of protein-ligand and DNA-ligand interactions in vitro is described. To test the reliability of the method, it was applied to the ES-MS analysis of protein-carbohydrate complexes...
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Initial Excited-State Structural Dynamics and Damage Kinetics of Nucleic Acid Derivatives and a Rhodopsin Analogue
DownloadFall 2013
Photochemical reactions resulting from the absorption of ultraviolet light are one of the main causes of DNA damage. For any excited-state photochemical reaction, it is the structural changes in the excited state after the absorption of the photon that ultimately decide the photochemical fate...
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Molecular Dynamics Simulations of Polyethylenimine Mediated Nucleic Acid Complexation with Implications for Non-viral Gene Delivery
DownloadSpring 2013
Gene therapy is a promising therapeutic technique that involves delivering nucleic acids into cells. Polycations have evolved into a major category of gene carriers. Polyethylenimine (PEI) is one of the most effective polycationic carriers. Furthermore, modifying PEI with certain lipophilic...
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2007
Berry, K.M., Erickson, T., Callander, D., Koss, R., Ngai, J., Stout, J., Waskiewicz, A.J., Wuennenberg-Stapleton, K., Moens, C.B., Hagey, D.W., French, C.R.
Background Pbx genes encode TALE class homeodomain transcription factors that pattern the developing neural tube, pancreas, and blood. Within the hindbrain, Pbx cooperates with Hox proteins to regulate rhombomere segment identity. Pbx cooperates with Eng to regulate midbrain-hindbrain boundary...
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Protein-glycolipid interactions studied in vitro using ESI-MS and nanodiscs: Insights into the mechanisms and energetics of binding
Download2015
Han, Ling Han, Kitova, Elena N., Klassen, John S., Pluvinage, Benjamin, Lin, Hong, Nikjah, Sanaz, Li, Jun, Boraston, Alisdair B.
Electrospray ionization-mass spectrometry (ESI-MS) analysis combined with the use of nanodiscs (NDs) to solubilize glycolipids (GLs) has recently emerged as a promising analytical method for detecting protein–GL interactions in vitro and, when applied to libraries of GLs, ranking their...