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Skip to Search Results- 1Alsaikhan, Bader H
- 1Araya, Luam Ellen
- 1Baghdasarian, Argishti
- 1Brennan, Lesley Jean
- 1Chan, Calvin J
- 1Chen, Lu
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Development and Applications of Stable Isotope Labelling Liquid Chromatography Mass Spectrometry for Quantitative Proteomics
DownloadSpring 2011
This thesis describes the characterization, automation, and applications of a stable isotope labelling method for quantitative proteomics by mass spectrometry. Known as 2MEGA, the method uses guanidinylation to convert peptide lysine residues to homoarginine followed by reductive methylation of...
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Fall 2012
The overall goal of this thesis research is to develop a spectral searching strategy capable of identifying peptide sequences from MS/MS spectra with high sensitivity and accuracy. First, a shotgun proteome analysis method was developed and successfully applied to the identification of proteins...
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Spring 2010
Interest in microfluidic platforms has surged as an alternative for sample preparation in the past two decades, with the potential for miniaturization, portability, automation, integration and parallelism driving this research. However, it is still very challenging to develop an integrated...
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Fall 2020
The Golgi complex resides at the center of the cellular trafficking pathway, where it functions in the modification, sorting, and trafficking of over one third of human proteins to their correct cellular destination. Initiation of vesicle formation at the Golgi requires activation of small...
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Fall 2021
Caspases are a family of enzymes that regulate biological processes, such as inflammation and programmed cell death, through proteolysis. For example, in the intrinsic pathway of apoptosis, cell death signaling involves cytochrome c release from the mitochondria, which leads to the activation of...
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Measuring proteomic changes associated with viral infections and neurodegenerative diseases using mass spectrometry
DownloadFall 2023
Viral infection and the resultant cellular stress can trigger significant changes to the cellular host proteome. Viral proteases essential to viral replication have been shown to cleave human host protein substrates as a mechanism of immune response evasion. The severe acute respiratory syndrome...
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Proteomic analysis of wheat (Triticum aestivum) whole roots and cell walls under water-deficit stress
DownloadSpring 2011
Wheat plants are affected by water-deficit stress in various regions of the world resulting in reduced crop productivity and thus decreased food production. To better understand the protein changes of water-deficit stress in wheat roots, comparative proteomics was performed using 2D gel...
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The Proteome of Partial Bladder Outlet Obstruction & The Therapeutic Role of Mesenchymal Stem Cells
DownloadFall 2013
Introduction: Partial bladder outlet obstruction (pBOO) is a common problem in urology. It passes through several pathological phases before progressing to bladder fibrosis. Herein, we investigated the proteomic profile of the urinary bladder in the early phase after pBOO, and examined the...
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Unique cellular interactions between the obligate intracellular bacteria Wolbachia pipientis and its insect host
DownloadFall 2011
Wolbachia are maternally inherited obligate intracellular bacteria found in arthropods, where they induce feminization, male-killing, parthenogenesis, and cytoplasmic incompatibility (CI). CI is conditional male sterility, in which Wolbachia-infected males successfully mate with infected females,...