The Proteome of Partial Bladder Outlet Obstruction & The Therapeutic Role of Mesenchymal Stem Cells

  • Author / Creator
    Alsaikhan, Bader H
  • Introduction:
    Partial bladder outlet obstruction (pBOO) is a common problem in urology. It passes through several pathological phases before progressing to bladder fibrosis. Herein, we investigated the proteomic profile of the urinary bladder in the early phase after pBOO, and examined the therapeutic role of mesenchymal stem cells (MSCs) in this pathology. Methods:
    Proteomic analysis of bladder tissue was performed using liquid- chromatography – mass spectrometry. The effects of intravenous injection of MSCs were examined on bladder function and gene transcription of previously described factors and newly detected proteins after proteomic analysis. Results:
    pBOO induced an increase in inflammatory, hypoxic, and cellular stress markers. Treatment with MSCs improved these molecular changes and helped maintain normal bladder capacity. Conclusion:
    Pathological changes happen early after pBOO. Inflammation, hypoxia, and mechanical strain seem to be the main factors in initiating the pathological cascade. Treatment with MSCs was successful in halting the inflammatory and hypoxic changes in the early phase after pBOO.

  • Subjects / Keywords
  • Graduation date
    Fall 2013
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
  • Specialization
    • Experimental Surgery
  • Supervisor / co-supervisor and their department(s)
  • Examining committee members and their departments
    • Churchill, Thomas A (Surgery)
    • Ding, Jie (Surgery)
    • Adesida, Adetola (Surgery)