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Skip to Search Results- 12Chen, Guanqun
- 11Xu, Yang
- 7Weselake, Randall J.
- 6Caldo, Kristian Mark P.
- 5Mietkiewska, Elzbieta
- 5Pan, Xue
- 33Graduate and Postdoctoral Studies (GPS), Faculty of
- 33Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 17Agricultural, Food and Nutritional Science, Department of
- 17Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 1Biological Sciences, Department of
- 1Biological Sciences, Department of/Journal Articles (Biological Sciences)
- 20Department of Agricultural, Food, and Nutritional Science
- 6Department of Biological Sciences
- 2Department of Agricultural, Food and Nutritional Science
- 1Department of Botany
- 1Department of Chemical Engineering
- 1Department of Food Science
- 3Deyholos, Michael (Biological Sciences)
- 2Habibur Rahman (Agricultural, Food and Nutritional Science)
- 2Rahman, Habibur (Agricultural, Food, and Nutritional Science)
- 1Allen Good (Biological Sciences)
- 1Ambrose, Divakar (Department of Agricultural, Food and Nutritional Science)
- 1Basu, Urmila (Department of Agricultural, Food & Nutritional Science)
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
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Identification of a pair of phospholipid:diacylglycerol acyltransferases from developing flax (Linum usitatissiumum L.) seed catalyzing the selective production of trilinolenin
Download2013
Wickramarathna, Aruna D., Siloto, Rodrigo M. P., Mietkiewska, Elzbieta, Pan, Xue, Weselake, Randall J.
The oil from flax (Linum usitatissimum L.) has high amounts of α-linolenic acid (ALA; 18:3cisΔ9,12,15) and is one of the richest sources of omega-3 polyunsaturated fatty acids (ω-3-PUFAs). To produce ∼57% ALA in triacylglycerol (TAG), it is likely that flax contains enzymes that can efficiently...
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2012
Kebede, Berisso, Rahman, Habibur
Further improving of seed yield, other agronomic and seed quality traits in spring canola Brassica napus requires broadening of genetic diversity in this crop. The European winter oilseed B. napus is known to be genetically diverse from spring oilseed B. napus. We hypothesized that elite spring...
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Improving Verticillium longisporum inoculation protocols and quantifying canola yield losses
DownloadSpring 2024
Verticillium stripe, caused by Verticillium longisporum, is an emerging soilborne disease of the Canadian canola (Brassica napus) crop. This study aimed to refine techniques for inoculating the pathogen on canola and to quantify its impact on hybrid canola yield under greenhouse and field...
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In vivo and in vitro evidence for biochemical coupling of reactions catalyzed by lysophosphatidylcholine acyltransferase and diacyglycerol acyltransferase
Download2015
Greer, Michael S., Pan, Xue, Weselake, Randall J., Chen, Guanqun, Zou, Jitao, Caldo, Kristian Mark P., Kazachkov, Michael
Seed oils of flax (Linum usitatissimum L.) and many other plant species contain substantial amounts of polyunsaturated fatty acids (PUFAs). Phosphatidylcholine (PC) is the major site for PUFA synthesis. The exact mechanisms of how these PUFAs are channeled from PC into triacylglycerol (TAG) needs...
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Introgression of Clubroot Resistance from Brassica oleracea into B. napus Canola through Interspecific Cross
DownloadSpring 2022
Clubroot disease caused by Plasmodiophora brassicae Woronin is a serious threat to canola production in Canada. Most of the available clubroot-resistant canola cultivars carry major resistance genes of the Brassica A genome. However, the ineffectiveness of this type of resistance has been...
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Kinetic improvement of an algal diacylglycerol acyltransferase 1 via fusion with an acyl-CoA binding protein
Download2020-01-01
Xu, Yang, Caldo, Kristian Mark P., Falarz, Lucas, Jayawardhane, Kethmi, Chen, Guanqun
SUMMARY Microalgal oils in the form of triacylglycerols (TAGs) are broadly used as nutritional supplements and biofuels. Diacylglycerol acyltransferase (DGAT) catalyzes the final step of acyl-CoA-dependent biosynthesis of TAG and is considered a key target for manipulating oil production....
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Molecular and functional characterization of sn-glycerol-3-phosphate acyltransferase of plants
DownloadSpring 2011
sn-Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the acylation of sn-1 position of sn-glycerol-3-phosphate to produce lysophosphatidic acid and Coenzyme A. GPATs are involved in several lipid synthetic pathways and play important physiological roles in plant development. The present...