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Accumulation of Al in Root Mucilage of an Al-Resistant and an Al-Sensitive Cultivar of Wheat
Download1996
Archambault, D. J., Zhang, G., Taylor, G. J.
To estimate rates of Al accumulation within the symplasm, all apoplastic pools of Al need to be eliminated or accounted for. We have developed a revised kinetic protocol that allows us to estimate the contribution of mucilage-bound Al to total, nonexchangeable Al, and to eliminate the mucilage as...
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Al-Induced, 51-Kilodalton, Membrane-Bound Proteins Are Associated with Resistance to Al in a Segregating Population of Wheat
Download1997
Zhang, G., Good, A., Taylor, G. J., Slaski, J. J., Basu, A.
Incorporation of S-35 into protein is reduced by exposure to Al in wheat (Triticum aestivum), but the effects are genotype-specific. Exposure to 10 to 75 mu M Al had little effect on S-35 incorporation into total protein, nuclear and mitochondrial protein, microsomal protein, and cytosolic...
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Amino acid polymorphisms in strictly conserved domains of a P-type ATPase HMA5 are involved in the mechanism of copper tolerance variation in Arabidopsis
Download2008
Kimura, K., Southron-Francis, J. L., Iuchi, S., Furuzawa, A., Taylor, G. J., Kobayashi, Y., Koyama, H., Kobayashi, M., Kuroda, K.
Copper (Cu) is an essential element in plant nutrition, but it inhibits the growth of roots at low concentrations. Accessions of Arabidopsis ( Arabidopsis thaliana) vary in their tolerance to Cu. To understand the molecular mechanism of Cu tolerance in Arabidopsis, we performed quantitative trait...
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Differential exudation of polypeptides by roots of aluminum-resistant and aluminum-sensitive cultivars of Triticum aestivum L. in response to aluminum stress
Download1994
Basu, A., Taylor, G. J., Basu, U.
Cultivars of Triticum aestivum differing in resistance to Al were grown under aseptic conditions in the presence and absence of Al and polypeptides present in root exudates were collected, concentrated, and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Upon exposure to...
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2000
Reid, R. J., Rengel, Z., McDonald-Stephens, J. L., Hunter, D. B., Elmore, D., Taylor, G. J., Bertsch, P. M.
Quantitative information on the uptake and distribution of Al at the cellular level is required to understand mechanisms of Al toxicity, but direct measurement of uptake across the plasma membrane has remained elusive. We measured rates of Al transport across membranes in single cells of Chara...
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2002
Zhang, G., Brachman, R. W. I., Robertson, P. K.
Abstract: An integrated approach to estimate liquefaction-induced ground settlements using CPT data for sites with level ground is presented. The approach combines an existing CPT-based method to estimate liquefaction resistance with laboratory test results on clean sand to evaluate the...
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2006
Wang, W., Li, J., Liu, D., Lu, Z., Zheng, H., Wong, G., Cai, Z., Fan, C., Zhang, J., Vang, S., Long, M., Zhang, G., Wang, J., Shi, J.
Retroposition is widely found to play essential roles in origination of new mammalian and other animal genes. However, the scarcity of retrogenes in plants has led to the assumption that plant genomes rarely evolve new gene duplicates by retroposition, despite abundant retrotransposons in plants...
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2007
Wang, W., Wang, H., Wong, G.K.S., Zheng, H., Clark, T., Zhang, G., Kang, L., Wang, J., Shi, J., Wang, X.
Background Insects constitute the vast majority of known species with their importance including biodiversity, agricultural, and human health concerns. It is likely that the successful adaptation of the Insecta clade depends on specific components in its proteome that give rise to specialized...
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Induction of microsomal membrane proteins in roots of an aluminum-resistant cultivar of Triticum aestivum L. under conditions of aluminum stress
Download1994
Basu, U., Basu, A., Taylor, G. J.
Three-day-old seedlings of an Al-sensitive (Neepawa) and an Al-resistant (PT741) cultivar of Triticum aestivum were subjected to Al concentrations ranging from a to 100 mu M for 72 h. At 25 mu M Al, growth of roots was inhibited by 57% in the Al-sensitive cultivar, whereas root growth in the...
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Induction of Vacuolar ATPase and Mitochondrial ATP Synthase by Aluminum in an Aluminum-Resistant Cultivar of Wheat
Download2001
Hamilton, C. A., Taylor, G. J., Good, A. G.
Two 51-kD aluminum (Al)-induced proteins (RMP51, root membrane proteins of 51 kD) were recently discovered in an aluminum-resistant cultivar of wheat (Triticum aestivum) cv PT741 (Basu et al., 1994a). These proteins segregate with the aluminum resistance phenotype in a segregating population...