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- 3Brassica napus
- 2Camelina sativa
- 2DGAT
- 2Diacylglycerol acyltransferase
- 2Saccharomyces-cerevisiae
- 3Xu, Yang
- 2Caldo, Kristian Mark P.
- 2Chen, Guanqun
- 2Weselake, Randall J.
- 1 Lemieux, M. Joanne
- 1Chen, Yongyan
- 7Graduate and Postdoctoral Studies (GPS), Faculty of
- 7Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
- 2Agricultural, Food and Nutritional Science, Department of
- 2Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
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Fall 2014
Quantitatively, nitrogen (N) has shown to be one of the most important nutrients for proper plant growth and development, and a key limiting factor in crop productivity. In the last 40 years, there has been a significant increase in the production of synthetic N-fertilizers, as well as an...
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Fall 2014
Monoamine oxidase enzymes are largely involved in the catabolism of biogenic amines. Two forms of the enzyme are socumented to exist, monoamine oxidase type A and B. The B form (MAO-B) of the enzyme has been noted to possess a high affinity site for some imidazoline ligands. This site (the I2...
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Engineering Enhanced Performance in Plant Diacylglycerol Acyltransferase and Long-Chain Acyl-CoA Synthetase
DownloadSpring 2018
Triacylglycerol (TAG) is the major storage lipid in higher plants and has great nutritional and industrial value. Plant storage lipid biosynthesis involves different subcellular compartments and a complex network of enzymes and proteins. Among them, endoplasmic reticulum (ER)-bound diacylglycerol...
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High-performance variants of plant diacylglycerol acyltransferase 1 generated by directed evolution provide insights into structure function
Download2017-09-10
Chen, Guanqun, Vanhercke, Thomas, Chen, Yongyan, Mietkiewska, Elzbieta, Siloto, Rodrigo M. P., Niesner, Nathalie, Xu, Yang, Caldo, Kristian Mark P., Weselake, Randall J., El Tahchy, Anna
Diacylglycerol acyltransferase 1 (DGAT1) catalyzes the acyl-CoA-dependent biosynthesis of triacylglycerol, the predominant component of seed oil. In some oil crops, including Brassica napus, the level of DGAT1 activity can have a substantial effect on triacylglycerol production....
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Multiple mechanisms contribute to increased neutral lipid accumulation in yeast producing recombinant variants of plant diacylglycerol acyltransferase 1
Download2017-09-01
Shah, Saleh, Xu, Yang, Ramakrishnan, Geetha, Caldo, Kristian Mark P., Chen, Guanqun, Ozga, Jocelyn, Greer, Michael S., Lemieux, M. Joanne, Wu, Limin, Weselake, Randall J.
The apparent bottleneck in the accumulation of oil during seed development in some oleaginous plant species is the formation of triacylglycerol (TAG) by the acyl-CoA-dependent acylation of sn-1,2- diacylglycerol catalyzed by diacylglycerol acyltransferase (DGAT, EC 2.3.1.20). Improving DGAT...
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Roles of Acyl-CoA:Diacylglycerol Acyltransferases 1 and 2 in Triacylglycerol Synthesis and Secretion in Hepatocytes
DownloadFall 2015
Triacylglycerol (TG) metabolism with its high-energy potential is stringently regulated. Unbalanced TG regulation, either too much TG that overwhelms cellular storage capacity or too little TG due to defects in adipogenesis, is associated with various metabolic disorders. TG can be exported from...