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Skip to Search Results- 10Steele, Michael A.
- 8AlZahal, Ousama
- 8McBride, Brian W.
- 5Dionissopoulos, Louis
- 3Laarman, Anne H.
- 3Plaizier, Jan C.
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A characterization of inflammatory and structural markers within the rumen epithelium during grain-induced ruminal acidosis in lactating dairy cattle
Download2012
Steele, Michael A., Plaizier, Jan C., AlZahal, Ousama, Dionissopoulos, Louis, McBride, Brian W.
The objective of this study was to characterize the mRNA and protein expression of inflammatory and structural genes in the rumen epithelium during grain-induced ruminal acidosis in lactating dairy cattle. A total of 16 rumen-fistulated, lactating Holstein dairy cattle (618±35 kg of body weight,...
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Adaptation to high grain diets proceeds through minimal immune system stimulation and differences in extracellular matrix protein expression in a model of subacute ruminal acidosis in non-lactating dairy cows
Download2012
AlZahal, Ousama, McBride, Brian W., Dionissopoulos, Louis, Steele, Michael A.
Problem statement: Subacute Ruminal Acidosis (SARA) is a metabolic disorder affecting approximately 20% of all dairy cattle in North America. Although the presence of SARA has been described for some time, the etiology of the disorder remains uncertain. For example, many animals diagnosed with...
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Butyrate and subacute ruminal acidosis affect abundance of membrane proteins involved with proton and short chain fatty acid transport in the rumen epithelium of dairy cows
Download2013
Steele, Michael A., Dionissopoulos, Louis, McBride, Brian W., AlZahal, Ousama, Greenwood, Sabrina L., Laarman, Anne H.
The objective of this study was to elucidate the effects of butyrate on the Short Chain Fatty Acids (SCFA) membrane transport proteins and proton membrane transport proteins in the rumen epithelium. Sixteen mid-lactation cows were fed a 44% Non-Fibre Carbohydrate (NFC) diet and divided into a...
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Butyrate-mediated genomic changes involved in the non-specific host defenses, matrix remodelling and the immune response in the rumen epithelium of cows afflicted with subacute ruminal acidosis
Download2013
Steele, Michael A., Greenwood, Sabrina L., Plaizier, Jan C., McBride, Brian W., Matthews, James C., Laarman, Anne H., AlZahal, Ousama, Dionissopoulos, Louis
Subacute Ruminal Acidosis (SARA) is a disorder in cattle which can lead to chronic inflammation in the rumen epithelium, known as rumenitis. Butyrate has been shown to attenuate some of the detrimental effects of inflammatory gastroenteral disorders but the molecular mechanisms mediated by...
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Changes in the rumen papillae during the periparturient transition in Holstein dairy cows are accompanied by changes in abundance of proteins involved in intracellular pH regulation, but not SCFA transport
Download2015
McBride, Brian W., Laarman, Anne H., Steele, Michael A., AlZahal, Ousama, Kleinberg, Adam
The objective of this study was to examine changes in SCFA transportcapacity in the rumen epithelium during the parturition transition period.Twelve pregnant, cannulated primiparous and multiparous cows were fed astandard Total Mixed Ration (TMR) for the duration of the dry period; aftercalving,...
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Development of ruminal and fecal microbiomes are affected by weaning but not weaning strategy in dairy calves
Download2016
Khafipour, Ehsan, Steele, Michael A., Meale, Sarah J., Plaizier, Jan C., Li, Shucong, Derakhshani, Hooman, Azevedo, Paula
The nature of weaning, considered the most stressful and significant transition experienced by dairy calves, influences the ability of a calf to adapt to the dramatic dietary shift, and thus, can influence the severity of production losses through the weaning transition. However, the effects of...
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2012
Steele, Michael A., Fisher, Rebecca E., Karrow, Niel A.
Adverse uterine environments experienced during fetal development can alter the projected growth pattern of various organs and systems of the body, leaving the offspring at an increased risk of metabolic disease. The thrifty phenotype hypothesis has been demonstrated as an alteration to the...
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Ruminal acidosis and the rapid onset of ruminal parakeratosis in a mature dairy cow: A case report
Download2009
Hook, Sarah E., AlZahal, Ousama, McBride, Brian W., Croom, Jim, Steele, Michael A.
A mature dairy cow was transitioned from a high forage (100% forage) to a high-grain (79% grain) diet over seven days. Continuous ruminal pH recordings were utilized to diagnose the severity of ruminal acidosis. Additionally, blood and rumen papillae biopsies were collected to describe the...
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2016
Steele, Michael A., Mitchell, C., AlZahal, Ousama, Or-Rashid, Mamun M., McBride, Brian W.
The objective of this experiment was to examine the effect of Subacute Ruminal Acidosis (SARA) on milk fatty acid (FA) profiles with emphasis on odd-chain and branched-chain FA. Sixteen multiparous Holstein dairy cows (618±35 kg of body weight, 221±32 days in milk) were used in a randomized...
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Transcriptomic changes in ruminal tissue induced by the periparturient transition in dairy cows
Download2014
Steele, Michael A., AlZahal, Ousama, Dionissopoulos, Louis, McBride, Brian W., Matthews, James C.
To understand how the capacity for fat metabolism (uptake, synthesis, modification) changes in rumen epithelia immediately before and after onset of lactation in dairy cows, rumen fluid Short Chain Fatty Acid (SCFA) concentrations and mRNA expression profiles of rumen epithelia was determined in...