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1996

Archambault, D. J., Zhang, G., Taylor, G. J.

To estimate rates of Al accumulation within the symplasm, all apoplastic pools of Al need to be eliminated or accounted for. We have developed a revised kinetic protocol that allows us to estimate the contribution of mucilage-bound Al to total, nonexchangeable Al, and to eliminate the mucilage as...

1997

Zhang, G., Good, A., Taylor, G. J., Slaski, J. J., Basu, A.

Incorporation of S-35 into protein is reduced by exposure to Al in wheat (Triticum aestivum), but the effects are genotype-specific. Exposure to 10 to 75 mu M Al had little effect on S-35 incorporation into total protein, nuclear and mitochondrial protein, microsomal protein, and cytosolic...

2008

Kimura, K., Southron-Francis, J. L., Iuchi, S., Furuzawa, A., Taylor, G. J., Kobayashi, Y., Koyama, H., Kobayashi, M., Kuroda, K.

Copper (Cu) is an essential element in plant nutrition, but it inhibits the growth of roots at low concentrations. Accessions of Arabidopsis ( Arabidopsis thaliana) vary in their tolerance to Cu. To understand the molecular mechanism of Cu tolerance in Arabidopsis, we performed quantitative trait...

1994

Basu, A., Taylor, G. J., Basu, U.

Cultivars of Triticum aestivum differing in resistance to Al were grown under aseptic conditions in the presence and absence of Al and polypeptides present in root exudates were collected, concentrated, and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Upon exposure to...

2000

Reid, R. J., Rengel, Z., McDonald-Stephens, J. L., Hunter, D. B., Elmore, D., Taylor, G. J., Bertsch, P. M.

Quantitative information on the uptake and distribution of Al at the cellular level is required to understand mechanisms of Al toxicity, but direct measurement of uptake across the plasma membrane has remained elusive. We measured rates of Al transport across membranes in single cells of Chara...

1994

Basu, U., Basu, A., Taylor, G. J.

Three-day-old seedlings of an Al-sensitive (Neepawa) and an Al-resistant (PT741) cultivar of Triticum aestivum were subjected to Al concentrations ranging from a to 100 mu M for 72 h. At 25 mu M Al, growth of roots was inhibited by 57% in the Al-sensitive cultivar, whereas root growth in the...

2001

Hamilton, C. A., Taylor, G. J., Good, A. G.

Two 51-kD aluminum (Al)-induced proteins (RMP51, root membrane proteins of 51 kD) were recently discovered in an aluminum-resistant cultivar of wheat (Triticum aestivum) cv PT741 (Basu et al., 1994a). These proteins segregate with the aluminum resistance phenotype in a segregating population...

1989

Zhang, G. C., Taylor, G. J.

Uptake of aluminum (Al) by excised roots of two Al-tolerant cultivars and two Al-sensitive cultivars of Triticum aestivum L. (wheat) was biphasic, with a rapid phase of uptake in the first 30 minutes followed by a linear phase of uptake up to 180 minutes. At the end of the uptake period, higher...

1990

Taylor, G. J., Shang, G. C.

The identity of a linear phase of aluminum (Al) uptake in Triticum aestivum was investigated by analysis of the kinetics of Al uptake by excised roots and purified cell wall fractions. Classical interpretation of kinetic data suggests that a linear phase of uptake with time reflects uptake across...

2003

Basu, U., Anoop, V. M., Taylor, G. J., McAlister-Henn, L., McCammon, M. T.

Aluminum (Al) toxicity is a major constraint for crop production in acid soils, although crop cultivars vary in their tolerance to Al. We have investigated the potential role of citrate in mediating Al tolerance in Al-sensitive yeast (Saccharomyces cerevisiae; MMYO11) and canola (Brassica napus...