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Approaches to Reverse Red Cell Sickling

  • Author / Creator
    Basonbul, Asmaa A
  • There is evidence that the symptoms associated with sickle cell disease can be relieved by using plant extracts containing anthocyanin (AC) agents. It has been established that ACs were able to reverse sickle cell morphology to a biconcave disc shape. However, no specific form of AC has been investigated in detail. In this thesis, a purified manufactured compound, delphinidin, was selected for study due to its antioxidant property. This study hypothesized that delphinidin chloride (Delph-CL) can reverse the sickle cell morphology by inhibiting hemoglobin S (Hb S) polymerization through a hydration and/or antioxidation biomechanism. Furthermore, the effect of Delph-CL on membrane integrity, Hb S solubility, and O2 binding were evaluated. Exposure to 100 µɡ/mL Delph-CL caused some sickle cells to transform into an elliptical shape (P = 0.04), possibly as a transition stage to discoid cells. Hb S polymerization also decreased which resulted in more O2 binding and a low P50. However, Delph-CL acted negatively on the red cell membrane by causing hemolysis. Neither one of the proposed biomechanisms were achieved and further work is necessary to establish the mechanism of sickle cell reversal.

  • Subjects / Keywords
  • Graduation date
    2016-06
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3KH0F45W
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Medical Sciences-Laboratory Medicine and Pathology
  • Supervisor / co-supervisor and their department(s)
    • Dr. Acker, Jason (Laboratory Medicine and Pathology)
    • Ms. Lew, Kristi (Laboratory Medicine and Pathology)
  • Examining committee members and their departments
    • Dr. Estey, Mathew (Laboratory Medicine and Pathology)
    • Dr. Onell, Rodrigo (Laboratory Medicine and Pathology)