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Engineering nitrogen use efficiency with alanine aminotransferase.
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- Author(s) / Creator(s)
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Nitrogen (N) is the most important factor limiting crop productivity worldwide. The ability of plants to acquire N from applied fertilizers is one of the critical steps limiting the efficient use of nitrogen. To improve N use efficiency, genetically modified plants that overexpress alanine aminotransferase (AlaAT) were engineered by introducing a barley AlaAT cDNA driven by a canola root specific promoter (btg26). Compared with wild-type canola, transgenic plants had increased biomass and seed yield both in the laboratory and field under low N conditions, whereas no differences were observed under high N.The transgenics also had increased nitrate influx. These changes resulted in a 40% decrease in the amount of applied nitrogen fertilizer required under field conditions to achieve yields equivalent to wild-type plants
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- Date created
- 2007
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- Type of Item
- Article (Published)