Genome-wide whole blood microRNAome and transcriptome analyses revealed miRNA-mRNA regulated host response to foodborne pathogen Salmonella infection in swine

• Author(s) / Creator(s)

  • Arantes, Adriano S.
  • Tuggle, Christopher K.
  • Guan, Le L.
  • Bearson, Shawn M.D.
  • Sun, Xu
  • Plastow, Graham S.
  • Kommadath, Arun
  • Bao, Hua
  • Stothard, Paul
  • Liang, Guanxiang

• To understand the role of miRNAs in regulating genes involved in host response to bacterial infection and shedding of foodborne pathogens, a systematic profiling of miRNAs and mRNAs from the whole blood of pigs upon Salmonella challenge was performed. A total of 62 miRNAs were differentially expressed post infection (false discovery rate <0.1). An integrative analysis of both the differentially expressed miRNAs and mRNAs using sequence-based miRNA target prediction and negative correlation of miRNA-mRNA profiles helped identify miRNA-mRNA networks that may potentially regulate host response to Salmonella infection. From these networks, miR-214 and miR-331-3p were identified as new candidates potentially associated with Salmonella infection. An miRNA seed sequence analysis suggested that these miRNAs regulate several critical immune-related genes including SLC11A1, PIGE-108A11.3 and VAV2. We showed that challenged pigs had reduced miR-214 expression and increased miR-331-3p expression in the whole blood. Furthermore, the expression of the proposed targets of miR-214 (SLC11A1 and PIGE-108A11.3) increased while that of the proposed target of miR-331-3p (VAV2) decreased following challenge (expression changes confirmed by in vitro assays). Based on these observations, we propose potential roles for miR-214 and miR-331-3p in regulation of immune responses to Salmonella infection.

• Date created

  2015-07-31

• Subjects / Keywords

  • Systems Biology
  • Regulatory Networks

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/R3TH8BR4F

• License

  Attribution 4.0 International