Using designed zinc finger proteins to inhibit hepatitis B virus transcription in tissue culture

  • Author / Creator
    Miller, Kristen L
  • Upon infection with hepatitis B virus (HBV) the result will be either a chronic infection or clearance of the virus. For the chronic carriers of HBV, therapy is primarily limited to nucleoside analogs, which inhibits viral replication and prevents production of progeny virus, however the nucleoside analogs have little affect on the reservoir of the virus in the nucleus, the covalently closed circular DNA (cccDNA). Our lab previously designed zinc finger proteins (ZFPs) to target sequences within the duck hepatitis B virus (DHBV) and HBV cccDNAs to inihibit hepadnaviral replication. This is a novel approach to treatment of HBV infection because the reservoir of the virus is directly targeted. The DHBV-specific ZFPs have been shown to inhibit DHBV replication in tissue culture. I have assessed the ability of HBV-specific ZFPs, delivered by lentiviral vectors, to inhibit HBV transcription in two HBV-infected human hepatoma cell lines. The currently designed ZFPs failed to suppress HBV replication.

  • Subjects / Keywords
  • Graduation date
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
    • Department of Medical Microbiology and Immunology
  • Specialization
    • Virology
  • Supervisor / co-supervisor and their department(s)
    • Tyrrell, D. Lorne (Medical Microbiology and Immunology)
  • Examining committee members and their departments
    • Tyrrell, D. Lorne (Medical Microbiology and Immunology)
    • Schang, Luis (Biochemistry)
    • Evans, David (Medical Microbiology and Immunology)
    • Houghton, Michael (Medical Microbiology and Immunology)