Usage
  • 63 views
  • 99 downloads

Expression and purification of recombinant forms of two Arabidopsis thaliana PR-10 homologues (MLP423 and Bet v 1)

  • Author / Creator
    MUHAMMAD, SAJAD
  • Pathogenesis related 10 (PR-10) proteins have important roles in mediating plant abiotic and biotic stress tolerance. Most of the members of this family possess ribonuclease activity. The homologues of PR-10 (ABR17) protein, MLP423 and Bet v 1, may have similar biological and physiological functions. The deduced amino acid sequence of MLP423 and Bet v 1, when compared to ABR17, indicated several conserved amino acids and a conserved P-loop motif (GxGGxGxxK). In the present investigation, cDNAs encoding Arabidopsis thaliana MLP423 and Bet v 1 were isolated and cloned, recombinant proteins expressed in E. coli, and purified using Ni-NTA and size exclusion chromatography. Both in-solution and in-gel RNase activity assays were conducted to determine whether these recombinant proteins possessed RNase activity, which indicated that neither MLP423 nor Bet v 1 possessed RNase activity. Our results are discussed in the context of the possible functions of these PR-10 homologues in plants.

  • Subjects / Keywords
  • Graduation date
    2013-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3901ZS6T
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Department of Agricultural, Food, and Nutritional Science
  • Specialization
    • Plant Science
  • Supervisor / co-supervisor and their department(s)
    • Professor Nat Kav, Department of Agricultural, Food and Nutritional Science, University of Alberta
  • Examining committee members and their departments
    • Professor Stephen Strelkov, Department of Agricultural, Food and Nutritional Science, University of Alberta
    • Professor Randall Weselake, Department of Agricultural, Food and Nutritional Science, University of Alberta
    • Professor Michael Deyholos, Department of Biological Sciences, University of Alberta
    • Professor Nat Kav, Department of Agricultural, Food and Nutritional Science, University of Alberta