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Broadening of genetic diversity in spring canola (Brassica napus L.) by use of C genome of B. oleracea var. capitata, and the effect of this on the performance of the inbred lines and their test-hybrids
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- Author / Creator
- Jani, Bhavikkumar N
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Broadening the genetic base of spring B. napus canola is important for continued progress in breeding of hybrid cultivars. The objectives of this study were to (i) assess the potential of the C genome of B. oleracea var. capitata (cabbage) for broadening the genetic base of the C genome of B. napus through B. napus × B. oleracea interspecific cross, (ii) investigate the prospect of developing euploid ‘canola’ lines from the F2 and BC1 (F1 × B. napus) plants, (iii) study the inheritance of B. oleracea SSR alleles in the interspecific-cross-derived inbred lines, and (iv) investigate the heterotic potential of these inbred lines by crossing to the spring B. napus parent as tester. Several canola quality inbred B. napus lines carrying allelic diversity introgressed from B. oleracea were developed through self-pollination of the interspecific hybrids. Analysis of the inbred lines with the C genome SSR markers indicated that the F2-derived lines carried a greater number of B. oleracea alleles as compared to the BC1-derived lines; however, the BC1-derived population retained a greater proportion of the theoretically expected number of alleles as compared to the F2-derived population. The mid-parent heterosis (MPH) for seed yield ranged from -22.5 to 43.3 % (mean 9.3 %) where the best performing hybrid showed 29 % improvement over the B. napus tester. Significant correlation was observed between the performance of the inbred lines and the test-hybrids; however, no correlation was found between the performance of the inbred lines and MPH. In general, the F2-derived population exhibited greater heterosis for seed yield as compared to the BC1-derived population. Correlation between genetic distance of the parents and MPH for seed yield was 0.31. Results from this study, thus, extend our knowledge of the value of the C genome of cabbage (B. oleracea) for exploitation in hybrid canola breeding
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- Graduation date
- Fall 2018
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- Type of Item
- Thesis
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- Degree
- Master of Science
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- License
- Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.