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Filtration of Glycoprotein-Modified Carboxylated Polystyrene Microspheres as Cryptosporidium Oocysts Surrogates: Effects of Flow Rate, Alum, and Humic Acid
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- Author(s) / Creator(s)
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In this study, a surrogate of Cryptosporidium parvum oocysts was prepared using Cryptosporidium-sized carboxylated polystyrene
microspheres and an alpha-1-acid glycoprotein (AGP). The zeta potential of the modified surrogates was found to be similar to
that of viable Cryptosporidium parvum. These surrogates were used in laboratory-scale filtration experiments under the effects of flow
velocity, alum addition, and humic acid (HA) coating on quartz sand surfaces. Filtration results showed that increased flow rates enhanced
the transport of microspheres mostly as a result of increased hydrodynamic force. HA adsorbed on the grain surfaces greatly enhanced the
mobility of microspheres in the packed bed, possibly as a result of increased repulsion between microspheres and the media. Alum inhibited
the transport of microspheres in the column mostly as a result of the charge neutralization of the microspheres. The transport of microspheres
when alum and HA coexisted was almost the same as the condition when alum and HAwere absent. Deposition profiles showed a monotonic
decline with distance under conditions where no HA was present, while nonmonotonic depositions were observed under conditions where
HA was adsorbed on collector surfaces. -
- Date created
- 2017-01-01
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- Subjects / Keywords
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- Type of Item
- Article (Published)