Filtration of Glycoprotein-Modified Carboxylated Polystyrene Microspheres as Cryptosporidium Oocysts Surrogates: Effects of Flow Rate, Alum, and Humic Acid

• Author(s) / Creator(s)

  • Huixin Zhang; Jeffrey Seaman; Yinan Wang; Hongbo Zeng; Ravin Narain; Ania Ulrich; Yang Liu

• In this study, a surrogate of Cryptosporidium parvum oocysts was prepared using Cryptosporidium-sized carboxylated polystyrene
  microspheres and an alpha-1-acid glycoprotein (AGP). The zeta potential of the modified surrogates was found to be similar to
  that of viable Cryptosporidium parvum. These surrogates were used in laboratory-scale filtration experiments under the effects of flow
  velocity, alum addition, and humic acid (HA) coating on quartz sand surfaces. Filtration results showed that increased flow rates enhanced
  the transport of microspheres mostly as a result of increased hydrodynamic force. HA adsorbed on the grain surfaces greatly enhanced the
  mobility of microspheres in the packed bed, possibly as a result of increased repulsion between microspheres and the media. Alum inhibited
  the transport of microspheres in the column mostly as a result of the charge neutralization of the microspheres. The transport of microspheres
  when alum and HA coexisted was almost the same as the condition when alum and HAwere absent. Deposition profiles showed a monotonic
  decline with distance under conditions where no HA was present, while nonmonotonic depositions were observed under conditions where
  HA was adsorbed on collector surfaces.

• Date created

  2017-01-01

• Subjects / Keywords

  • Cryptosporidium; Surrogate; Porous media; Transport; Flow rate; Humic acid

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/r3-vwen-b322

• License

  Attribution-NonCommercial 4.0 International