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The Sensitivity of Canadian Wheat Genotypes to the Host-Selective Toxins Produced by Pyrenophora tritici-repentis
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- Author / Creator
- Tran,Van Anh, Thi
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Tan spot is a foliar disease of wheat caused by the fungus Pyrenophora tritici–repentis. Disease development is associated with the production of three host-selective toxins (Ptr ToxA, Ptr ToxB, and Ptr ToxC) that serve as fungal pathogenicity factors. The aim of this study was to investigate the sensitivity to each of these toxins in a representative collection of 100 Canadian wheat cultivars known as the VarComp population. Heterologously expressed, His-tagged Ptr ToxA and Ptr ToxB, and spore germination fluids possessing Ptr ToxC activity, were produced and used to screen the cultivars for toxin sensitivity. Ninety two of the 100 wheat cultivars tested were sensitive to at least one toxin, with 68% found to be sensitive to Ptr ToxA, 63% sensitive to Ptr ToxC, and 24% sensitive to Ptr ToxB. Eight cultivars were insensitive to all three toxins and may represent an important resource for tan spot resistance breeding activities.
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- Subjects / Keywords
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- Graduation date
- Fall 2014
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- Type of Item
- Thesis
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- Degree
- Master of Science
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- License
- This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.