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Inhibition of Listeria monocytogenes growth and biofilm production by nisin and phenolic compounds.
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- Author / Creator
- Li, Kuan-Lin
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The synergistic antimicrobial and anti-biofilm activities of nisin and phenolic compounds against Listeria monocytogenes were investigated. Among the twenty six phenolic compounds tested in this study, butyl gallate, octyl gallate, lauryl gallate, propyl gallate, naringenin and quercetin exhibited the most potent antimicrobial activity in L. monocytogenes with minimal inhibitory concentration (MIC) lower than 512 μg/ml. Butyl gallate was the only phenolic compound that exhibited synergistic inhibitory activity against all tested strains of L. monocytogenes in combination with nisin. In addition, nisin showed anti-biofilm activity against L. monocytogenes ATCC 19115 at concentrations as low as 10 IU/ml. Naringenin, propyl gallate, octyl gallate and lauryl gallate at concentrations as low as 5 μg/ml increased the anti-biofilm activity of nisin at10 IU/ml. The enhanced anti-biofilm activity of nisin in combination with naringenin, propyl gallate, octyl gallate and lauryl gallate were also observed in different strains of L. monocytogenes. Fluorescent microscopic imaging demonstrated that biofilm formation by L. monocytogenes ATCC 19115 was inhibited in the presence of nisin alone, and with nisin and naringenin. These results suggest that phenolic compounds can be used as effective adjuvant to increase the ant-listerial and anti-biofilm activity of nisin.
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- Graduation date
- Fall 2017
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- Type of Item
- Thesis
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- Degree
- Master of Science
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- License
- This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.