Studying the effects of mouse prion protein on mice and rat colon antibacterial gene expression profiling

  • Author / Creator
    Lam, Tran Hu Th
  • The objectives of this thesis research are: 1) to evaluate whether there is interspecies translocation of mouse recombinant prion protein (moPrP) through colon tissue of rats; 2) to investigate whether lipopoplysaccharide (LPS) from Escherichia coli influences permeability of moPrP through the colon tissue of mice; 3) to assess whether mouse (mo)PrP affects antibacterial gene expression profile in the colon of rats and mice; 4) to test whether combinations of moPrP with LPS or detoxified LPS (D-LPS) are able to affect genes related to antibacterial response; 5) to examine whether presence of LPS on the mucosal or serosal side of the colon tissues will affect permeability of moPrP and gene expression profiles in the colon. Overall, moPrP alone or in combination with LPS or D-LPS did not translocate through the intact colon tissues of rats or mice. moPrP alone or combined with LPS or D-LPS on the mucosal or serosal sides of diffusion chamber affected expression of various genes related to inflammation and antibacterial response.

  • Subjects / Keywords
  • Graduation date
    Fall 2013
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.