Whole genome sequence analysis of an alachlor and endosulfan degrading Pseudomonas strain W15Feb9B isolated from Ochlockonee River, Florida

• Author(s) / Creator(s)

  • Ewida, Ayman Y. I.
  • Stothard, Paul
  • Chauhan, Ashvini
  • Griffiths, Zabrenna
  • Pathak, Ashish

• We recently isolated a Pseudomonas sp. strain W15Feb9B from Ochlockonee River, Florida and demonstrated potent biodegradative activity against two commonly used pesticides - Alachlor [(2-chloro-2′,6′-diethylphenyl-N (methoxymethyl)acetanilide)] and Endosulfan [(6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9methano-2,3,4-benzo(e)di-oxathiepin-3-oxide], respectively. To further identify the repertoire of metabolic functions possessed by strain W15Feb9B, a draft genome sequence was obtained, assembled, annotated and analyzed. The genome sequence of strain 2385 has been deposited in GenBank under accession number JTKF00000000; BioSample number SAMN03151543. The sequences obtained from strain 2385 assembled into 192 contigs with a genome size of 6,031,588, G + C content of 60.34, and 5512 total number of putative genes. RAST annotated a total of 542 subsystems in the genome of strain W15Feb9B along with the presence of 5360 coding sequences. A genome wide survey of strain W15Feb9B indicated that it has the potential to degrade several other pollutants including atrazine, caprolactam, dioxin, PAHs (such as naphthalene) and several chloroaromatic compounds.

• Date created

  2016

• Subjects / Keywords

  • Pseudomonas
  • Whole Genome Sequencing (WGS)
  • Biodegradation
  • Alachlor
  • Endosulfan

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/R3SF2MR4G

• License

  Attribution-NonCommerical-NoDerivs 4.0 International