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Asialofetuin-coated PLGA Nanoparticles for Targeting Hepatocytes

  • Author / Creator
    Kharaud, Gagandeep
  • The purpose of this project was to formulate, modify and evaluate nanoparticles for targeting asialoglycoprotein receptor found in large numbers exclusively on hepatocyte surfaces. Our goal is to increase the efficacy, reduce the side effects and the cost of the hydrophilic drugs administered to cure diseases like hepatitis C virus. Selective drug delivery into targeted cells using nanoparticles is one effective approach to enhance activity and avoid systemic side effects. Here we describe our effort towards the development of specially engineered poly(D,L-lactic-co-glycolic acid) nanoparticles using double emulsion method and surface coat them with asialofetuin for targeted delivery into hepatocytes. Bovine Serum Albumin-coated nanoparticles were prepared as a negative control. Furthermore, covalently conjugated protein on nanoparticle was labeled with rhodamine and was used for cell-based studies. Results from these studies indicated that asialofetuin conjugated with nanoparticles showed enhanced and selective uptake by hepatocytes compared to nanoparticles conjugated with Bovine Serum Albumin.

  • Subjects / Keywords
  • Graduation date
    2010-11
  • Type of Item
    Thesis
  • Degree
    Masters of Science in Pharmaceutical Sciences
  • DOI
    https://doi.org/10.7939/R32K8C
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Faculty of Pharmacy and Pharmaceutical Sciences
  • Supervisor / co-supervisor and their department(s)
    • Kaur, Kamaljit (Faculty of Pharmacy and Pharmaceutical Sciences)
  • Examining committee members and their departments
    • Suresh, Mavanur (Faculty of Pharmacy and Pharmaceutical Sciences)
    • Lavasanifar, Afsaneh (Faculty of Pharmacy and Pharmaceutical Sciences)
    • Uludag, Hasan (Chemical and Materials Engineering, Faculty of Engineering)