Preparation of chitooligosaccharide from fungal waste mycelium by recombinant chitinase

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  • This study aimed to develop an enzymatic method for conversion of chitin from fungal waste mycelia to chitooligosaccharides. The recombinant chitinase LlChi18A from Lactococcus lactis was over-expressed by Escherichia coli BL21 (DE3) and purified by affinity chromatography. The enzymatic properties of the purified enzyme were studied by chitin oligosaccharides. Waste mycelium was pre-treated by alkaline. The optimal conditions for hydrolysis of fungal chitin by recombinant chitinase were determined by Schales method. HPLC/ESI-MS was used to determine the content of N-acetylglucosamine and chitooligosaccharides after hydrolysis. The level of reducing sugar released from pretreated mycelium by chitinase increased with the reaction time during 6 days. The main product in the hydrolysates was N,N′-diacetylchitobiose. After hydrolysis by chitinase for 5 d, the yield of N,N′-diacetylchitobiose from waste mycelium was around 10% with estimated purity of around 70%. Combination of chitinase and snailase remarkably increased the yield to 24% with purity of 78%. Fungal mycelium which contains chitin is a new potential source for obtaining food grade chitooligosaccharides.

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    Article (Draft / Submitted)
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    Attribution-NonCommercial-NoDerivatives 4.0 International
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    • Lv, M., Hu, Y., Gänzle, M. G., Lin, J., Wang, C., & Cai, J. (2016). Preparation of chitooligosaccharide from fungal waste mycelium by recombinant chitinase. Carbohydrate Research, 430, 1-7.
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