Preparation of chitooligosaccharide from fungal waste mycelium by recombinant chitinase

• Author(s) / Creator(s)

  • Lv, Mengyuan
  • Hu, Ying
  • Gänzle, Michael G.
  • Lin, Jianguo
  • Wang, Changgao
  • Cai, Jun

• This study aimed to develop an enzymatic method for conversion of chitin from fungal waste mycelia to chitooligosaccharides. The recombinant chitinase LlChi18A from Lactococcus lactis was over-expressed by Escherichia coli BL21 (DE3) and purified by affinity chromatography. The enzymatic properties of the purified enzyme were studied by chitin oligosaccharides. Waste mycelium was pre-treated by alkaline. The optimal conditions for hydrolysis of fungal chitin by recombinant chitinase were determined by Schales method. HPLC/ESI-MS was used to determine the content of N-acetylglucosamine and chitooligosaccharides after hydrolysis. The level of reducing sugar released from pretreated mycelium by chitinase increased with the reaction time during 6 days. The main product in the hydrolysates was N,N′-diacetylchitobiose. After hydrolysis by chitinase for 5 d, the yield of N,N′-diacetylchitobiose from waste mycelium was around 10% with estimated purity of around 70%. Combination of chitinase and snailase remarkably increased the yield to 24% with purity of 78%. Fungal mycelium which contains chitin is a new potential source for obtaining food grade chitooligosaccharides.

• Date created

  2016-01-01

• Subjects / Keywords

  • Waste Mycelium
  • Chitinase
  • Chitin
  • Hydrolysis
  • N,N′-Diacetylchitobiose

• Type of Item

  Article (Draft / Submitted)

• DOI

  https://doi.org/10.7939/r3-ab1d-6p79

• License

  Attribution-NonCommercial-NoDerivatives 4.0 International