Non digestible carbohydrates in the diet determine toxicity of irinotecan (CPT-11)/5-fluorouracil in rats independently of β-glucuronidase activity in intestinal lumen

  • Author / Creator
    Farhangfar, Arazm
  • Aim of this study was to assess effects of non-digestible carbohydrates on the pathobiology of mucosal injury after chemotherapy. Rats bearing colon tumor (n=6/diet) were fed diets including 8% (w/w) as either cellulose, inulin, fructo-oligosaccharide (OligoF), 50:50 (w/w) inulin and oligoF (Synergy®), isomalto-oligosaccharide (IMO), type IV resistant starch (RS4) or cornstarch. Animals were treated with 2 cycles of CPT-11/5-fluorouracil. Animals fed RS4 or Synergy® were least ill with the least weight loss and anorexia, highest levels of gut associated lymphoid tissue (GALT) CD3+ and CD3CD8+ cells, least acute phase responses (haptoglobin, α-1-acid glycoprotein), least GALT OX6+ cells, CD152+ and CD71+ expression on CD8+ cells. Animals fed IMO or cornstarch were the most ill by these same criteria (p<0.05); animals fed inulin, cellulose and OligoF showed intermediate sickness. Dietary NDCHO determine chemotherapy toxicity in a controlled model of colon cancer treatment, this may be due to change in immune function.

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  • Type of Item
  • Degree
    Master of Science
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