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Detection and Characterization of Orthoreovirus in Alberta's Environmental Waters

  • Author / Creator
    Kostiuk, Tyler T.
  • Reoviruses have been shown to infect a very broad range of mammalian sources and infections in humans have been associated with gastrointestinal and respiratory illnesses. While the presence of reovirus in several different water matrices and clinical samples has been reported, the clinical implications of reovirus in Alberta’s water and clinical samples have not been studied because of the lack of an appropriate screening method. To improve reovirus detection, a novel reverse-transcription real time PCR (qRT-PCR) assay was designed, developed, and implemented. Two sets of novel qPCR primers and probes (targeting the M1 and L3 genetic fragments) were designed and found to have sensitivities of 5 genetic copies per qPCR for M1 and 50 genetic copies per qPCR for L1, respectively. Using the newly developed qRT-PCR assay, reoviruses were detected in wastewater (15/16) and environmental surface water (20/216) samples obtained from various sites in Alberta, while no reoviruses were observed in clinical fecal samples obtained from patients suffering from gastroenteritis. Phylogenetic trees produced from S4 gene fragment sequences indicate that a variety of S4 alleles are present in the environment in Alberta, which is consistent with data previously published regarding reoviruses in the environment.

  • Subjects / Keywords
  • Graduation date
    2015-11
  • Type of Item
    Thesis
  • Degree
    Master of Science
  • DOI
    https://doi.org/10.7939/R3V97ZX40
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Master's
  • Department
    • Medical Sciences-Laboratory Medicine and Pathology
  • Supervisor / co-supervisor and their department(s)
    • Neumann, Norman (Public Health)
    • Pang, Xiao-Li (Laboratory Medicine and Pathology)
  • Examining committee members and their departments
    • Neumann, Normann (Public Health)
    • Pang, Xiao-Li (Laboratory Medicine and Pathology)
    • Lee, Bonita (Pediatrics)
    • Preiksaitis, Jutta (Medicine)
    • Shmulevitz, Maya (Medical Microbiology and Immunology)